HLA class II sequence-based typing in normal Saudi individuals

: We have adopted a system that combines low resolution PCR‐SSP followed by sequence‐based typing (SBT) to analyze HLA‐DRB1, ‐DPB1 and ‐DQB1 alleles in the Saudi population. The SBT method was used to identify HLA class II alleles in Saudis for the first time. Nineteen HLA‐DRB1 alleles in currently...

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Bibliographic Details
Published in:Tissue antigens 2002-09, Vol.60 (3), p.259-261
Main Authors: Al-Hussein, K.A., Rama, N.R., Butt, A.I., Meyer, B., Rozemuller, E., Tilanus, M.G.J.
Format: Article
Language:English
Subjects:
Arabs - genetics
Gene Frequency
Histocompatibility Antigens Class II - genetics
Histocompatibility Testing
HLA
Humans
PCR-SSP
Saudi Arabia
SBT
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Summary:: We have adopted a system that combines low resolution PCR‐SSP followed by sequence‐based typing (SBT) to analyze HLA‐DRB1, ‐DPB1 and ‐DQB1 alleles in the Saudi population. The SBT method was used to identify HLA class II alleles in Saudis for the first time. Nineteen HLA‐DRB1 alleles in currently recognized subtypes of the DRB locus were detected. DR1 and DR9 were not encountered. SBT did not detect diversity within the DR7 and DR10 alleles. Sixteen HLA‐DQB1 and 10 HLA‐DPB1 alleles were identified. This study represents the first molecular report on the HLA class II allele frequency in the population of Saudi Arabia.
ISSN:0001-2815
1399-0039
DOI:10.1034/j.1399-0039.2002.600308.x