Molecular diagnostic procedures for production of pathogen-free propagation material

• Published in: Pest management science 2002-11, Vol.58 (11), p.1126-1131
• Main Authors: Manulis, Shulamit, Chalupowicz, Laura, Dror, Orit, Kleitman, Frida
• Format: Article
• Language: English
• Subjects: Agrobacterium radiobacter; Agrobacterium tumefaciens; Agrobacterium tumefaciens - genetics; Agrobacterium tumefaciens - growth & development; Agrobacterium tumefaciens - pathogenicity; Aster Plant - microbiology; bacteria; Bacteria - genetics; Bacteria - growth & development; Bacteria - pathogenicity; bacterial diseases of plants; bacterial plant pathogen; Bacterial plant pathogens; Biological and medical sciences; Brassicaceae; Brassicaceae - microbiology; Caryophyllaceae - microbiology; clones; detection; DNA primers; DNA, Bacterial - genetics; Erwinia - genetics; Erwinia - growth & development; Erwinia - pathogenicity; Erwinia herbicola pv gypsophilae; Fundamental and applied biological sciences. Psychology; Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control; genes; Geranium - microbiology; Magnoliopsida - microbiology; microbial detection; molecular biology; pathogenicity; pest management; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - microbiology; plant identification; plant pathogens; polymerase chain reaction; propagation materials; Random Amplified Polymorphic DNA Technique; rapid methods; Rosa; Rosa - microbiology; Specific Pathogen-Free Organisms; Xanthomonas campestris - genetics; Xanthomonas campestris - growth & development; Xanthomonas campestris - pathogenicity; Xanthomonas campestris pv campestris; Xanthomonas campestris pv pelargonii
• ISSN: 1526-498X; 1526-4998
• DOI: 10.1002/ps.533

Production of disease-free propagation material is a major means of controlling most bacterial diseases of plants, particularly when neither resistant clones nor effective chemical treatments are available. For this purpose sensitive, specific and rapid detection methods are required. The advent of molecular biology and, in particular, the polymerase chain reaction (PCR) has opened new ways for the characterization and identification of plant pathogens and the development of disease-management strategies. PCR-based detection methods rely on the development of primers for the specific detection of the pathogen. The use of pathogenicity genes as targets for primer design is the preferred procedure for obtaining specific primers but other procedures may also be useful for this purpose. In the present review we describe four examples of procedures for detecting four important bacterial pathogens in Israel: Erwinia herbicola pv gypsophilae in gypsophila, Xanthomonas campestris pv pelargonii in geranium, Agrobacterium tumefaciens in asters and roses, and Xanthomonas campestris pv campestris in crucifers. Procedures for constructing specific PCR primers for each bacterium are illustrated and discussed as well as the combination of PCR with other methods.