Glutamatergic Signaling through the N-Methyl-d-Aspartate Receptor Directly Activates Medial Subpopulations of Luteinizing Hormone-Releasing Hormone (LHRH) Neurons, But Does Not Appear to Mediate the Effects of Estradiol on LHRH Gene Expression

• Published in: Endocrinology (Philadelphia) 2002-12, Vol.143 (12), p.4837-4845
• Main Authors: Ottem, Erich N, Godwin, Jonathan G, Petersen, Sandra L
• Format: Article
• Language: English
• Subjects: 17β-Estradiol; Animal models; Animals; Dizocilpine Maleate - pharmacology; Estradiol - pharmacology; Excitatory Amino Acid Antagonists - pharmacology; Female; Gene expression; Gene Expression - drug effects; Glutamate receptors; Glutamatergic transmission; Glutamic acid receptors (ionotropic); Glutamine - metabolism; Gonadotropin-releasing hormone; Gonadotropin-Releasing Hormone - analysis; Gonadotropin-Releasing Hormone - genetics; Gonadotropin-Releasing Hormone - physiology; Luteinizing hormone; N-Methyl-D-aspartic acid receptors; N-Methylaspartate - pharmacology; Neurons; Neurons - physiology; Nuclei; Ovariectomy; Preoptic area; Preoptic area (medial); Preoptic Area - cytology; Rats; Rats, Sprague-Dawley; Receptors; Receptors, N-Methyl-D-Aspartate - drug effects; Receptors, N-Methyl-D-Aspartate - genetics; Receptors, N-Methyl-D-Aspartate - physiology; RNA, Messenger - analysis; Sex hormones; Signal Transduction; Steroid hormones; Subpopulations; Tissue Distribution
• ISSN: 0013-7227; 1945-7170
• DOI: 10.1210/en.2002-220707

Although estradiol (E2) triggers phasic increases in LH-releasing hormone (LHRH) synthesis and release, the neurocircuitry responsible for these changes is unclear. We used an ovariectomized, E2-treated animal model to investigate the possibility that glutamate, through N-methyl-d-aspartate (NMDA) receptors (NMDAR), communicates E2 signals to LHRH neurons. A neuroanatomical analysis of the region containing LHRH neurons revealed that approximately 80% of LHRH neurons in medial, but less than 40% in lateral, nuclei of the preoptic area contained NMDAR1 mRNA. Consistent with this distribution pattern, NMDA doubled LHRH mRNA levels in medial neurons, but increased them by less than 30% in cells of the lateral nuclei. Steroids did not alter NMDAR1 mRNA levels in LHRH neurons or change the percentage of LHRH neurons expressing the gene. Furthermore, in contrast to the regionalized effects of NMDA, E2 treatment increased LHRH mRNA levels to the same extent in medial and lateral neurons, and MK801 failed to block E2-induced changes in LHRH gene expression. These results demonstrate that glutamatergic signaling via NMDA receptors is direct and preferentially targets LHRH neurons in medial nuclei of the preoptic area. However, it is unlikely that NMDAR activation mediates E2-dependent increases in LHRH mRNA levels before the LH surge.