Antisense down regulation of NtBI-1 in tobacco BY-2 cells induces accelerated cell death upon carbon starvation

Bax inhibitor-1 (BI-1) protein is proposed to be a conserved programmed cell death suppressor. In this report, we investigate the anti-apoptotic function of plant BI-1 by antisense (AS) down regulation of NtBI-1 in Nicotiana tabacum cv. BY-2 cells. We observed that AS cell lines were more susceptibl...

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Bibliographic Details
Published in:FEBS letters 2002-12, Vol.532 (1), p.111-114
Main Authors: Bolduc, Nathalie, Brisson, Louise F
Format: Article
Language:English
Subjects:
(p, h)BI-1, (plant, human) Bax inhibitor-1
Apoptosis
AS, antisense
Autophagy
Bax inhibitor-1
Cell Line
DNA Fragmentation
DNA, Antisense - genetics
DNA, Plant - analysis
Down-Regulation
GUS, β-glucuronidase
Kinetics
Man, mannitol
Membrane Proteins - genetics
Membrane Proteins - metabolism
Membrane Proteins - physiology
Nicotiana - cytology
Nicotiana - growth & development
PCD, programmed cell death
Plant
Programmed cell death
Suc, sucrose
Sucrose - metabolism
TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling
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Summary:Bax inhibitor-1 (BI-1) protein is proposed to be a conserved programmed cell death suppressor. In this report, we investigate the anti-apoptotic function of plant BI-1 by antisense (AS) down regulation of NtBI-1 in Nicotiana tabacum cv. BY-2 cells. We observed that AS cell lines were more susceptible to autophagy, internucleosomal DNA fragmentation and death than control cells when subjected to sucrose starvation and hypo-osmotic shock, in agreement with a role of BI-1 as a death inhibitor.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(02)03650-5