Automated sample preparation by pressurized liquid extraction–solid-phase extraction for the liquid chromatographic–mass spectrometric investigation of polyphenols in the brewing process

The analysis of polyphenols from solid plant or food samples usually requires laborious sample preparation. The liquid extraction of these compounds from the sample is compromised by apolar matrix interferences, an excess of which has to be eliminated prior to subsequent purification and separation....

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Bibliographic Details
Published in:Journal of Chromatography A 2002-11, Vol.976 (1), p.345-348
Main Authors: Papagiannopoulos, Menelaos, Mellenthin, Annett
Format: Article
Language:English
Subjects:
Automation
Beers
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Fermented food industries
Flavonoids
Food industries
Fundamental and applied biological sciences. Psychology
Humulus - chemistry
Mass Spectrometry - methods
Phenols - analysis
Polymers - analysis
Polyphenols
Reproducibility of Results
Spectrophotometry, Ultraviolet
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Summary:The analysis of polyphenols from solid plant or food samples usually requires laborious sample preparation. The liquid extraction of these compounds from the sample is compromised by apolar matrix interferences, an excess of which has to be eliminated prior to subsequent purification and separation. Applying pressurized liquid extraction to the extraction of polyphenols from hops, the use of different solvents sequentially can partly overcome these problems. Initial extraction with pentane eliminates hydrophobic compounds like hop resins and oils and enables the straightforward automated on-line solid-phase extraction as part of an optimized LC–MS analysis.
ISSN:0021-9673
DOI:10.1016/S0021-9673(02)01230-X