Proliferative activity and expression of cyclin-dependent kinase inhibitor p21WAF1 and p53 protein in endothelial cells of human aorta during replicative aging in vitro

Experiments with bromodeoxyuridine showed that the count of nonproliferating cells in a monolayer culture of aortic endothelial cells from adult humans rapidly increased during long-term subculturing. Cytochemical assay showed that these cells contain neutral b-galactosidase, the marker of aging cel...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2002-07, Vol.134 (1), p.81-83
Main Authors: Antropova, Yu G, Bryantsev, A L, Kalinina, N I, Il'inskaya, O P, Tararak, E M
Format: Article
Language:English
Subjects:
Adult
Aging
Aorta - cytology
Aorta - metabolism
beta-Galactosidase - metabolism
Bromodeoxyuridine - pharmacology
Cell Division
Cell Nucleus - metabolism
Cyclin-Dependent Kinase Inhibitor p21
Cyclins - biosynthesis
Endothelium, Vascular - cytology
Endothelium, Vascular - metabolism
Humans
Immunohistochemistry
In Vitro Techniques
Reactive Oxygen Species
Time Factors
Tumor Suppressor Protein p53 - biosynthesis
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Summary:Experiments with bromodeoxyuridine showed that the count of nonproliferating cells in a monolayer culture of aortic endothelial cells from adult humans rapidly increased during long-term subculturing. Cytochemical assay showed that these cells contain neutral b-galactosidase, the marker of aging cells. Immunocytochemical assay demonstrated that most cells express p53 protein and inhibitor of the cell cycle p21WAF1.
ISSN:0007-4888
1573-8221