Alkane hydroxylase homologues in Gram-positive strains

Summary We isolated Gram‐positive alkane‐degraders from soil and a tricking‐bed reactor, and show using polymerase chain reaction (PCR) with degenerate alkane hydroxylase primers and Southern blots that most Rhodococcus isolates contain three to five quite divergent homologues of the Pseudomonas put...

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Bibliographic Details
Published in:Environmental microbiology 2002-11, Vol.4 (11), p.676-682
Main Authors: Van Beilen, Jan B., Smits, Theo H. M., Whyte, Lyle G., Schorcht, Susanne, Röthlisberger, Martina, Plaggemeier, Thorsten, Engesser, Karl-Heinrich, Witholt, Bernard
Format: Article
Language:English
Subjects:
Alkanes - metabolism
Blotting, Southern
Cytochrome P-450 CYP4A
Cytochrome P-450 Enzyme System - metabolism
Gram-Positive Bacteria - classification
Gram-Positive Bacteria - enzymology
Mixed Function Oxygenases - metabolism
Phylogeny
Polymerase Chain Reaction
Soil Microbiology
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Summary:Summary We isolated Gram‐positive alkane‐degraders from soil and a tricking‐bed reactor, and show using polymerase chain reaction (PCR) with degenerate alkane hydroxylase primers and Southern blots that most Rhodococcus isolates contain three to five quite divergent homologues of the Pseudomonas putida GPo1 alkB gene. Two Mycobacterium isolates each contain one homologue, however there is no evidence for the presence of alkB homologues in the remaining strains.
ISSN:1462-2912
1462-2920
DOI:10.1046/j.1462-2920.2002.00355.x