Nonlinear bio‐photonic crystal effects revealed with multimodal nonlinear microscopy

Summary Highly optically active nonlinear bio‐photonic crystalline and semicrystalline structures in living cells were studied by a novel multimodal nonlinear microscopy. Numerous biological structures, including stacked membranes and aligned protein structures are highly organized on a nanoscale an...

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Bibliographic Details
Published in:Journal of microscopy (Oxford) 2002-12, Vol.208 (3), p.190-200
Main Authors: Chu, S.‐W., Chen, I.‐H., Liu, T.‐M., Sun, C.‐K., Lee, S.‐P., Lin, B.‐L., Cheng, P.‐C., Kuo, M.‐X., Lin, D.‐J., Liu, H.‐L.
Format: Article
Language:English
Subjects:
Animals
Brain
Cell Physiological Phenomena
Cells - ultrastructure
Cr: forsterite laser
Crystallization
Humans
Mice
Microscopy, Confocal - instrumentation
Microscopy, Confocal - methods
multiphoton process
Muscle, Skeletal - ultrastructure
Nonlinear Dynamics
nonlinear microscopy
Optics and Photonics
photonic crystal
Photons
Plant Cells
second‐harmonic generation
Skin
Swine
third harmonic generation
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Summary:Summary Highly optically active nonlinear bio‐photonic crystalline and semicrystalline structures in living cells were studied by a novel multimodal nonlinear microscopy. Numerous biological structures, including stacked membranes and aligned protein structures are highly organized on a nanoscale and have been found to exhibit strong optical activities through second‐harmonic generation (SHG) interactions, behaving similarly to man‐made nonlinear photonic crystals. The microscopic technology used in this study is based on a combination of different imaging modes including SHG, third‐harmonic generation, and multiphoton‐induced fluorescence. With no energy release during harmonic generation processes, the nonlinear‐photonic‐crystal‐like SHG activity is useful for investigating the dynamics of structure–function relationships at subcellular levels and is ideal for studying living cells, as minimal or no preparation is required.
ISSN:0022-2720
1365-2818
DOI:10.1046/j.1365-2818.2002.01081.x