Genetics of xanthan production in Xanthomonas campestris: the xanA and xanB genes are involved in UDP-glucose and GDP-mannose biosynthesis

The nucleotide sequence of a 3.4-kb EcoRI-PstI DNA fragment of Xanthomonas campestris pv. campestris revealed two open reading frames, which were designated xanA and xanB. The genes xanA and xanB encode proteins of 448 amino acids (molecular weight of 48,919) and 466 amino acids (molecular weight of...

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Bibliographic Details
Published in:Journal of Bacteriology 1992-01, Vol.174 (1), p.191-199
Main Authors: Koplin, R. (Universitat Bielefeld, Bielefeld, Germany), Arnold, W, Hotte, B, Simon, R, Wang, G, Puhler, A
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
Amino Acid Sequence
Bacteria
Bacteriology
Base Sequence
Biological and medical sciences
BIOSINTESIS
BIOSYNTHESE
Biotechnology
Carbohydrate Sequence
ENZIMAS
ENZYME
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genetic Complementation Test
Genetics
GLUCOSA
GLUCOSE
Glucosyltransferases - biosynthesis
Glucosyltransferases - genetics
GOMA XANTAN
Guanosine Diphosphate Mannose - biosynthesis
Guanosine Diphosphate Mannose - genetics
MANNOSE
Mannose-6-Phosphate Isomerase - genetics
MANOSA
Microbiology
Molecular Sequence Data
NUCLEOTIDE
NUCLEOTIDOS
Nucleotidyltransferases - genetics
Polysaccharides, Bacterial - biosynthesis
Polysaccharides, Bacterial - genetics
Pseudomonas aeruginosa
Reading Frames
Sequence Homology, Nucleic Acid
XANTHANE
XANTHOMONAS CAMPESTRIS
Xanthomonas campestris - enzymology
Xanthomonas campestris - genetics
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Summary:The nucleotide sequence of a 3.4-kb EcoRI-PstI DNA fragment of Xanthomonas campestris pv. campestris revealed two open reading frames, which were designated xanA and xanB. The genes xanA and xanB encode proteins of 448 amino acids (molecular weight of 48,919) and 466 amino acids (molecular weight of 50,873), respectively. These genes were identified by analyzing insertion mutants which were known to be involved in xanthan production. Specific tests for the activities of enzymes involved in the biosynthesis of UDP-glucose and GDP-mannose indicated that the xanA gene product was involved in the biosynthesis of both glucose 1-phosphate and mannose 1-phosphate. The deduced amino acid sequence of xanB showed a significant degree of homology (59%) to the phosphomannose isomerase of Pseudomonas aeruginosa, a key enzyme in the biosynthesis of alginate. Moreover, biochemical analysis and complementation experiments with the Escherichia coli manA fragment revealed that xanB encoded a bifunctional enzyme, phosphomannose isomerase-GDP-mannose pyrophosphorylase
ISSN:0021-9193
1098-5530
1067-8832
DOI:10.1128/jb.174.1.191-199.1992