Identification of low-abundance differentially expressed transcripts using arrayed cDNA clones

Analysis of comparative gene expression by the use of DNA microarrays has become a widely used tool. However, this technique is only readily applied to organisms where sequence information is known. This paper describes the development of a low-cost method of gene discovery by enrichment of differen...

Full description

Saved in:
Bibliographic Details
Published in:Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2002-12, Vol.133 (4), p.537-542
Main Authors: Golby, P, Stephens, S.K, Rast, J.P, Burke, J.F
Format: Article
Language:English
Subjects:
Animals
DNA array
DNA, Complementary
Drosophila melanogaster - genetics
Drosophila melanogaster - metabolism
Drosophila Proteins - genetics
Drosophila Proteins - metabolism
Female
Filter
Gene discovery
Gene expression
Gene Expression Profiling
Gene Library
Macroarray
Male
Membrane
Nucleic Acid Hybridization
Nylons
Oligonucleotide Array Sequence Analysis - methods
Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Subtractive hybridisation
Transcription, Genetic
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Analysis of comparative gene expression by the use of DNA microarrays has become a widely used tool. However, this technique is only readily applied to organisms where sequence information is known. This paper describes the development of a low-cost method of gene discovery by enrichment of differentially expressed transcripts, which uses cDNA library arrays of bacterial clones on nylon membranes (macroarrays) coupled with a subtractive probe preparation method to discover differentially expressed genes. The method requires no prior knowledge of the organism's genome sequence and overcomes the inherent insensitivity of standard methods of macroarray hybridisation.
ISSN:1096-4959
1879-1107
DOI:10.1016/S1096-4959(02)00146-X