Effect of activators and inhibitors on the activity of mitochondrial DNA polymerase

At a concentration of 0.5 to 3 mmol/l, ATP stimulates the activity of mitochondrial DNA polymerase of Neurospora crassa under the optimum reaction conditions; at higher concentrations, an inhibitory effect is observed. 4-Chloromercuribenzoate (1 mmol/L), a thiol inhibitor, decreases the enzyme activ...

Full description

Saved in:
Bibliographic Details
Published in:Folia microbiologica 1991-01, Vol.36 (3), p.229-233
Main Authors: GRONES, J, NOVOTNY, C
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - pharmacology
Biological and medical sciences
Dideoxynucleotides
DNA-Directed DNA Polymerase - metabolism
Enzyme Activation - drug effects
Ethidium - pharmacology
Fundamental and applied biological sciences. Psychology
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Heparin - pharmacology
Microbiology
Mitochondria - enzymology
Mycology
Neurospora crassa
Neurospora crassa - drug effects
Neurospora crassa - enzymology
Nucleic Acid Synthesis Inhibitors
Sulfhydryl Reagents - pharmacology
Thymine Nucleotides - pharmacology
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:At a concentration of 0.5 to 3 mmol/l, ATP stimulates the activity of mitochondrial DNA polymerase of Neurospora crassa under the optimum reaction conditions; at higher concentrations, an inhibitory effect is observed. 4-Chloromercuribenzoate (1 mmol/L), a thiol inhibitor, decreases the enzyme activity two-fold, while N-ethylmalcimide (2 mmol/L) has no effect. Ethidium bromide (up to 10 mumol/L) and heparin (up to 0.4 micrograms/mL) reduce the activity by 60%. ddTTP does not affect the DNA polymerase reaction. The best in vitro template is the activated calf-thymus DNA.
ISSN:0015-5632
1874-9356
DOI:10.1007/BF02814353