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Effect of activators and inhibitors on the activity of mitochondrial DNA polymerase
At a concentration of 0.5 to 3 mmol/l, ATP stimulates the activity of mitochondrial DNA polymerase of Neurospora crassa under the optimum reaction conditions; at higher concentrations, an inhibitory effect is observed. 4-Chloromercuribenzoate (1 mmol/L), a thiol inhibitor, decreases the enzyme activ...
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| Published in: | Folia microbiologica 1991-01, Vol.36 (3), p.229-233 |
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| Main Authors: | , |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c301t-ffc542ea7442b5d3aaf8113111798e55e318f880e48f09dc5e3b3d2503aa42273 |
| container_end_page | 233 |
| container_issue | 3 |
| container_start_page | 229 |
| container_title | Folia microbiologica |
| container_volume | 36 |
| creator | GRONES, J NOVOTNY, C |
| description | At a concentration of 0.5 to 3 mmol/l, ATP stimulates the activity of mitochondrial DNA polymerase of Neurospora crassa under the optimum reaction conditions; at higher concentrations, an inhibitory effect is observed. 4-Chloromercuribenzoate (1 mmol/L), a thiol inhibitor, decreases the enzyme activity two-fold, while N-ethylmalcimide (2 mmol/L) has no effect. Ethidium bromide (up to 10 mumol/L) and heparin (up to 0.4 micrograms/mL) reduce the activity by 60%. ddTTP does not affect the DNA polymerase reaction. The best in vitro template is the activated calf-thymus DNA. |
| doi_str_mv | 10.1007/BF02814353 |
| format | article |
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Ethidium bromide (up to 10 mumol/L) and heparin (up to 0.4 micrograms/mL) reduce the activity by 60%. ddTTP does not affect the DNA polymerase reaction. The best in vitro template is the activated calf-thymus DNA.</description><identifier>ISSN: 0015-5632</identifier><identifier>EISSN: 1874-9356</identifier><identifier>DOI: 10.1007/BF02814353</identifier><identifier>PMID: 1841856</identifier><identifier>CODEN: FOMIAZ</identifier><language>eng</language><publisher>Praha: Academia</publisher><subject>Adenosine Triphosphate - pharmacology ; Biological and medical sciences ; Dideoxynucleotides ; DNA-Directed DNA Polymerase - metabolism ; Enzyme Activation - drug effects ; Ethidium - pharmacology ; Fundamental and applied biological sciences. Psychology ; Growth, nutrition, metabolism, transports, enzymes. Molecular biology ; Heparin - pharmacology ; Microbiology ; Mitochondria - enzymology ; Mycology ; Neurospora crassa ; Neurospora crassa - drug effects ; Neurospora crassa - enzymology ; Nucleic Acid Synthesis Inhibitors ; Sulfhydryl Reagents - pharmacology ; Thymine Nucleotides - pharmacology</subject><ispartof>Folia microbiologica, 1991-01, Vol.36 (3), p.229-233</ispartof><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c301t-ffc542ea7442b5d3aaf8113111798e55e318f880e48f09dc5e3b3d2503aa42273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5186622$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1841856$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GRONES, J</creatorcontrib><creatorcontrib>NOVOTNY, C</creatorcontrib><title>Effect of activators and inhibitors on the activity of mitochondrial DNA polymerase</title><title>Folia microbiologica</title><addtitle>Folia Microbiol (Praha)</addtitle><description>At a concentration of 0.5 to 3 mmol/l, ATP stimulates the activity of mitochondrial DNA polymerase of Neurospora crassa under the optimum reaction conditions; at higher concentrations, an inhibitory effect is observed. 4-Chloromercuribenzoate (1 mmol/L), a thiol inhibitor, decreases the enzyme activity two-fold, while N-ethylmalcimide (2 mmol/L) has no effect. Ethidium bromide (up to 10 mumol/L) and heparin (up to 0.4 micrograms/mL) reduce the activity by 60%. ddTTP does not affect the DNA polymerase reaction. The best in vitro template is the activated calf-thymus DNA.</description><subject>Adenosine Triphosphate - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Dideoxynucleotides</subject><subject>DNA-Directed DNA Polymerase - metabolism</subject><subject>Enzyme Activation - drug effects</subject><subject>Ethidium - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</subject><subject>Heparin - pharmacology</subject><subject>Microbiology</subject><subject>Mitochondria - enzymology</subject><subject>Mycology</subject><subject>Neurospora crassa</subject><subject>Neurospora crassa - drug effects</subject><subject>Neurospora crassa - enzymology</subject><subject>Nucleic Acid Synthesis Inhibitors</subject><subject>Sulfhydryl Reagents - pharmacology</subject><subject>Thymine Nucleotides - pharmacology</subject><issn>0015-5632</issn><issn>1874-9356</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNqF0D1PwzAQBmALgUopLOxIGRADUsDnj9gd29ICUgUDMEeOY6tGSVzsFKn_npRUdGQ63b2PbngRugR8BxiL--kCEwmMcnqEhiAFS8eUZ8doiDHwlGeUnKKzGD8xzjCjZIAGIBlIng3R29xao9vE20Tp1n2r1oeYqKZMXLNyhftdfZO0K9MD1253uO4SvfJNGZyqkoeXSbL21bY2QUVzjk6sqqK52M8R-ljM32dP6fL18Xk2WaaaYmhTazVnxCjBGCl4SZWyEoACgBhLw7mhIK2U2DBp8bjU3aGgJeG4k4wQQUfopv-7Dv5rY2Kb1y5qU1WqMX4Tc0EEJ0LKfyFkjBPGWQdve6iDjzEYm6-Dq1XY5oDzXdX5oeoOX-2_boralAfad9vl1_tcRa0qG1SjXfxjHGSWEUJ_AHe8hF4</recordid><startdate>19910101</startdate><enddate>19910101</enddate><creator>GRONES, J</creator><creator>NOVOTNY, C</creator><general>Academia</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19910101</creationdate><title>Effect of activators and inhibitors on the activity of mitochondrial DNA polymerase</title><author>GRONES, J ; NOVOTNY, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-ffc542ea7442b5d3aaf8113111798e55e318f880e48f09dc5e3b3d2503aa42273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Adenosine Triphosphate - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Dideoxynucleotides</topic><topic>DNA-Directed DNA Polymerase - metabolism</topic><topic>Enzyme Activation - drug effects</topic><topic>Ethidium - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</topic><topic>Heparin - pharmacology</topic><topic>Microbiology</topic><topic>Mitochondria - enzymology</topic><topic>Mycology</topic><topic>Neurospora crassa</topic><topic>Neurospora crassa - drug effects</topic><topic>Neurospora crassa - enzymology</topic><topic>Nucleic Acid Synthesis Inhibitors</topic><topic>Sulfhydryl Reagents - pharmacology</topic><topic>Thymine Nucleotides - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GRONES, J</creatorcontrib><creatorcontrib>NOVOTNY, C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Folia microbiologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GRONES, J</au><au>NOVOTNY, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of activators and inhibitors on the activity of mitochondrial DNA polymerase</atitle><jtitle>Folia microbiologica</jtitle><addtitle>Folia Microbiol (Praha)</addtitle><date>1991-01-01</date><risdate>1991</risdate><volume>36</volume><issue>3</issue><spage>229</spage><epage>233</epage><pages>229-233</pages><issn>0015-5632</issn><eissn>1874-9356</eissn><coden>FOMIAZ</coden><abstract>At a concentration of 0.5 to 3 mmol/l, ATP stimulates the activity of mitochondrial DNA polymerase of Neurospora crassa under the optimum reaction conditions; at higher concentrations, an inhibitory effect is observed. 4-Chloromercuribenzoate (1 mmol/L), a thiol inhibitor, decreases the enzyme activity two-fold, while N-ethylmalcimide (2 mmol/L) has no effect. 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| identifier | ISSN: 0015-5632 |
| ispartof | Folia microbiologica, 1991-01, Vol.36 (3), p.229-233 |
| issn | 0015-5632 1874-9356 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72752788 |
| source | Springer Online Journal Archives (Through 1996) |
| subjects | Adenosine Triphosphate - pharmacology Biological and medical sciences Dideoxynucleotides DNA-Directed DNA Polymerase - metabolism Enzyme Activation - drug effects Ethidium - pharmacology Fundamental and applied biological sciences. Psychology Growth, nutrition, metabolism, transports, enzymes. Molecular biology Heparin - pharmacology Microbiology Mitochondria - enzymology Mycology Neurospora crassa Neurospora crassa - drug effects Neurospora crassa - enzymology Nucleic Acid Synthesis Inhibitors Sulfhydryl Reagents - pharmacology Thymine Nucleotides - pharmacology |
| title | Effect of activators and inhibitors on the activity of mitochondrial DNA polymerase |
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