System for combined three-dimensional morphological and molecular analysis of thick tissue specimens

We present a new system for simultaneous morphological and molecular analysis of thick tissue samples. The system is composed of a computer‐assisted microscope and a JAVA‐based image display, analysis, and visualization program that allows acquisition, annotation, meaningful storage, three‐dimension...

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Bibliographic Details
Published in:Microscopy research and technique 2002-12, Vol.59 (6), p.522-530
Main Authors: Fernandez-Gonzalez, Rodrigo, Jones, Arthur, Garcia-Rodriguez, Enrique, Chen, Ping Yuan, Idica, Adam, Lockett, Stephen J., Barcellos-Hoff, Mary Helen, Ortiz-De-Solorzano, Carlos
Format: Article
Language:English
Subjects:
3D reconstruction
Animals
assisted histopathology
Breast Neoplasms - chemistry
Breast Neoplasms - pathology
computer assisted microscopy
Female
Humans
Image Cytometry - instrumentation
Image Cytometry - methods
Image Processing, Computer-Assisted - methods
JAVA
Mice
Microcomputers
Microscopy, Confocal - instrumentation
Microscopy, Confocal - methods
Oncogene Proteins - analysis
Oncogene Proteins - genetics
Software
Space life sciences
User-Computer Interface
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Summary:We present a new system for simultaneous morphological and molecular analysis of thick tissue samples. The system is composed of a computer‐assisted microscope and a JAVA‐based image display, analysis, and visualization program that allows acquisition, annotation, meaningful storage, three‐dimensional reconstruction, and analysis of structures of interest in thick sectioned tissue specimens. We describe the system in detail and illustrate its use by imaging, reconstructing, and analyzing two complete tissue blocks that were differently processed and stained. One block was obtained from a ductal carcinoma in situ (DCIS) lumpectomy specimen and stained alternatively with Hematoxilyn and Eosin (H&E), and with a counterstain and fluorescence in situ hybridization (FISH) to the ERB‐B2 gene. The second block contained a fully sectioned mammary gland of a mouse, stained for histology with H&E. We show how the system greatly reduces the amount of interaction required for the acquisition and analysis and is, therefore, suitable for studies that require morphologically driven, wide‐scale (e.g., whole gland) analysis of complex tissue samples or cultures. Microsc. Res. Tech. 59:522–530, 2002. Published 2002 Wiley‐Liss, Inc.
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.10233