Expression regulation of the endochitinase chit36 from Trichoderma asperellum (T. harzianum T-203)

The presence of the endochitinase CHIT36 from Trichoderma harzianum TM was assessed in several antagonistic Trichoderma strains belonging to different molecular taxonomic groups. CHIT37 from T. harzianum CECT 2413 was sequenced and found to display 89% homology with CHIT36 at the amino acid level. N...

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Bibliographic Details
Published in:Current genetics 2002-11, Vol.42 (2), p.114-122
Main Authors: Viterbo, Ada, Montero, Manuel, Ramot, Ofir, Friesem, Dana, Monte, Enrique, Llobell, Antonio, Chet, Ilan
Format: Article
Language:English
Subjects:
Alternaria - drug effects
Alternaria - physiology
Amino Acid Sequence
Botrytis - drug effects
Botrytis - physiology
Chitinases - genetics
Chitinases - metabolism
Chitinases - pharmacology
Fungal Proteins - pharmacology
Fusarium - drug effects
Fusarium - physiology
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Fungal
Green Fluorescent Proteins
Hexosaminidases - pharmacology
Luminescent Proteins - genetics
Luminescent Proteins - metabolism
Molecular Sequence Data
Nitrogen - metabolism
Pichia - genetics
Promoter Regions, Genetic
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Rhizoctonia - genetics
Sequence Homology, Amino Acid
Spores, Fungal - drug effects
Transformation, Genetic
Trichoderma - enzymology
Trichoderma - genetics
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Summary:The presence of the endochitinase CHIT36 from Trichoderma harzianum TM was assessed in several antagonistic Trichoderma strains belonging to different molecular taxonomic groups. CHIT37 from T. harzianum CECT 2413 was sequenced and found to display 89% homology with CHIT36 at the amino acid level. Northern analysis showed that chit36Y from T. asperellum is regulated both by glucose and nitrogen levels. Stress conditions, colloidal chitin and N-acetyl-glucosamine are effective inducers of this gene. The promoter of chit36Y was cloned and was used to direct expression of a gfp reporter gene in Trichoderma transformants. Confrontation experiments with the plant pathogen Rhizoctonia solani revealed that direct contact between the fungi is not necessary for gfp expression. The R. solani-inducing factor appears to be a soluble molecule capable of diffusing through a dialysis membrane (
ISSN:0172-8083
1432-0983
DOI:10.1007/s00294-002-0345-4