Helicobacter Species Ribosomal DNA Recovered from the Liver Tissue of Chinese Patients with Primary Hepatocellular Carcinoma

Using polymerase chain reaction (PCR), we examined 16S ribosomal DNA (rDNA) of Helicobacter species in liver tissue specimens obtained from 15 patients with hepatocellular carcinoma. Sixty percent (9 of 15) of these specimens were found to be positive for Helicobacter species. Four 16S rDNA fragment...

Full description

Saved in:
Bibliographic Details
Published in:Clinical infectious diseases 2002-12, Vol.35 (12), p.1555-1557
Main Authors: Fan, Xue-Gong, Peng, Xiao-Ning, Huang, Yan, Yakoob, Javed, Wang, Zhi-Ming, Chen, Yong-Ping
Format: Article
Language:English
Subjects:
Adult
Asian Continental Ancestry Group
Biological and medical sciences
Brief Reports
Carcinoma, Hepatocellular - microbiology
Cirrhosis
DNA, Bacterial - analysis
DNA, Ribosomal - analysis
Female
Gastroenterology. Liver. Pancreas. Abdomen
Helicobacter
Helicobacter pylori
Helicobacter pylori - genetics
Helicobacter pylori - isolation & purification
Hepatocellular carcinoma
Humans
Infections
Liver
Liver Neoplasms - microbiology
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Male
Medical sciences
Middle Aged
Polymerase Chain Reaction
Predisposing factors
Ribosomal DNA
RNA, Ribosomal, 16S - analysis
RNA, Ribosomal, 16S - genetics
Specimens
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Using polymerase chain reaction (PCR), we examined 16S ribosomal DNA (rDNA) of Helicobacter species in liver tissue specimens obtained from 15 patients with hepatocellular carcinoma. Sixty percent (9 of 15) of these specimens were found to be positive for Helicobacter species. Four 16S rDNA fragments from positive PCR samples were directly sequenced. By sequence comparison, all were found to be 99% identical to the 16S rRNA of Helicobacter pylori.
ISSN:1058-4838
1537-6591
DOI:10.1086/344770