The essential Drosophila melanogaster gene wds (will die slowly) codes for a WD-repeat protein with seven repeats

We have isolated and characterized the will die slowly (wds) gene of Drosophila melanogaster, formerly known as l(1)zw8 or l(1)3Ad. The gene codes for a 2.0-kb RNA that is transcribed at all stages of development. The RNA has been localized by in situ hybridization to imaginal discs, larval brain, t...

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Bibliographic Details
Published in:Molecular genetics and genomics : MGG 2002-12, Vol.268 (4), p.425-433
Main Authors: Hollmann, M, Simmerl, E, Schäfer, U, Schäfer, M A
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Blotting, Northern
Chromosome Mapping
Chromosomes
Cloning
Cloning, Molecular
Conserved Sequence - genetics
DNA, Complementary - genetics
Drosophila melanogaster - genetics
Drosophila Proteins
Female
Gene Expression Regulation, Developmental
Genes, Essential
Genomes
In Situ Hybridization
Insect Proteins - genetics
Insect Proteins - isolation & purification
Insect Proteins - metabolism
Insects
Male
Molecular Sequence Data
Proteins
Protocol
Repetitive Sequences, Nucleic Acid - genetics
RNA, Messenger - analysis
Sequence Homology, Amino Acid
Signal transduction
Transcription, Genetic
X Chromosome - genetics
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Summary:We have isolated and characterized the will die slowly (wds) gene of Drosophila melanogaster, formerly known as l(1)zw8 or l(1)3Ad. The gene codes for a 2.0-kb RNA that is transcribed at all stages of development. The RNA has been localized by in situ hybridization to imaginal discs, larval brain, to nurse cells in the ovary, and to spermatogonia and spermatocytes in the testis. The putative translation product contains seven WD-repeats and is, therefore, a new member of the family of WD-proteins. Clear homologues of the Drosophila WDS protein exist in three other fully sequenced higher eukaryotes - human, Caenorhabditis elegans and Arabidopsis. A genomic fragment containing the wds transcription unit is able to rescue two different lethal wds alleles, thus proving that we have indeed isolated the wds gene.
ISSN:1617-4615
1617-4623
DOI:10.1007/s00438-002-0768-0