Sequence analysis of STR polymorphisms at locus ACTBP2 in the Taiwanese population

A highly polymorphic sequence structure is reported in the human beta-actin related pseudogene 2 (ACTBP2) (SE33) locus in members of the Taiwanese Han population. A total of 100 unrelated members of the Taiwanese Han population were used in the study. Alleles that shared the same size but differ in...

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Bibliographic Details
Published in:Forensic science international 2002-12, Vol.130 (2), p.112-121
Main Authors: Hsieh, Hsing-Mei, Wu, Kun-Long, Tsai, Li-Chin, Lo, Chia-Hsin, Linacre, Adrian, Lee, James Chun-I
Format: Article
Language:English
Subjects:
ACTBP2
Actin
Alleles
Amplification
Biological and medical sciences
Conformation
DNA Fingerprinting - methods
Electrophoresis
Electrophoresis, Polyacrylamide Gel
Forensic medicine
Gels
Gene Frequency
Gene polymorphism
Genetic Markers
Genotype
Genotype & phenotype
Genotypes
Humans
Medical sciences
Paternity
Paternity testing
PCR
Polymerase Chain Reaction
Polymorphism
Polymorphism, Genetic
Population genetics
Public health. Hygiene
Public health. Hygiene-occupational medicine
Sequence analysis
Sequence Analysis, DNA
Single-stranded DNA
SSCP
STR
Taiwan - ethnology
Tandem Repeat Sequences
Tropical medicine
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Summary:A highly polymorphic sequence structure is reported in the human beta-actin related pseudogene 2 (ACTBP2) (SE33) locus in members of the Taiwanese Han population. A total of 100 unrelated members of the Taiwanese Han population were used in the study. Alleles that shared the same size but differ in their sequence are described to allow for inter laboratory sharing of data. PCR products amplified from this locus were separated by single-strand conformation polymorphism electrophoresis, the single-stranded DNA bands were excised from the gels, a second amplification performed, and then the PCR products were sequenced. All the alleles differed by either 2 or 4 bp. Sequence variations were observed as deletions or insertions in the repeat units AG (or AA) and AAAG. Additionally, transitions in the flanking regions were recorded. A total of 27 alleles with 71 associated genotypes were recorded if the alleles were defined by size, but 68 alleles with 88 associated genotypes were noted with the alleles were scored on the basis of sequence variation. The power of discrimination (Pd) of this single locus was 0.9874 making the human ACTBP2 a good alternative marker for individual identification and paternity testing.
ISSN:0379-0738
1872-6283
DOI:10.1016/S0379-0738(02)00350-X