Influence of repeated lyophilization on the survival of Deinococcus proteolyticus, Micrococcus luteus and Escherichia coli

Repeated lyophilization of Deinococcus proteolyticus, Micrococcus luteus and Escherichia coli cells results in a successive decrease of their survival. The survival curve is exponential with E. coli and M. luteus, and sigmoidal with a broad shoulder with D. proteolyticus both after repeated lyophili...

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Bibliographic Details
Published in:Folia microbiologica 1991-01, Vol.36 (1), p.71-74
Main Authors: RYZNAR, L, DRAZIL, V
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
DNA, Bacterial - genetics
DNA, Bacterial - radiation effects
Escherichia coli
Escherichia coli - cytology
Escherichia coli - genetics
Escherichia coli - radiation effects
Freeze Drying
Freezing
Fundamental and applied biological sciences. Psychology
Gamma Rays
Microbiology
Micrococcus - cytology
Micrococcus - genetics
Micrococcus - radiation effects
Micrococcus luteus
Micrococcus luteus - cytology
Micrococcus luteus - genetics
Micrococcus luteus - radiation effects
Radiation Tolerance
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Summary:Repeated lyophilization of Deinococcus proteolyticus, Micrococcus luteus and Escherichia coli cells results in a successive decrease of their survival. The survival curve is exponential with E. coli and M. luteus, and sigmoidal with a broad shoulder with D. proteolyticus both after repeated lyophilization and after UV- or gamma-irradiation. When cells were subjected to gamma-irradiation after a 20-fold freeze-drying, the corresponding survival curve became exponential without the shoulder. Hence we assume that irradiation and repeated lyophilization afflict the same cellular structures and/or functions.
ISSN:0015-5632
1874-9356
DOI:10.1007/BF02935825