Aerotactic responses in bacteria to photoreleased oxygen

Abstract Bacterial aerotaxis is a rapid response towards or away from oxygen. Here we report on the use of computer-assisted motion analysis coupled to flash photolysis of caged oxygen to quantify aerotactic responses in bacteria. The caged compound (µ-peroxo)(µ-hydroxo)bis[bis(bipyridyl) cobalt(III...

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Bibliographic Details
Published in:FEMS microbiology letters 2002-12, Vol.217 (2), p.237-242
Main Authors: Yu, Hyung Suk, Saw, Jimmy H., Hou, Shaobin, Larsen, Randy W., Watts, Kylie J., Johnson, Mark S., Zimmer, Michael A., Ordal, George W., Taylor, Barry L., Alam, Maqsudul
Format: Article
Language:English
Subjects:
Aerotaxis
Bacillus subtilis - growth & development
Bacillus subtilis - metabolism
Bacteria
Bacterial Physiological Phenomena
Bacteriology
Binding Sites
Biological and medical sciences
Cage compounds
Caged oxygen
Capillary tubes
Cobalt
Cobalt compounds
E coli
Escherichia coli - growth & development
Escherichia coli - metabolism
Flash photolysis
Fundamental and applied biological sciences. Psychology
Irradiation
Microbiology
Motility, taxis
Motion analysis
Movement
Mutation
Oxygen
Oxygen - metabolism
Oxygen compounds
Perchlorate
Perchloric acid
Photochemistry
Photolysis
Signal processing
Signal transduction
Spectrophotometry, Ultraviolet
Transducers
Ultraviolet radiation
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Summary:Abstract Bacterial aerotaxis is a rapid response towards or away from oxygen. Here we report on the use of computer-assisted motion analysis coupled to flash photolysis of caged oxygen to quantify aerotactic responses in bacteria. The caged compound (µ-peroxo)(µ-hydroxo)bis[bis(bipyridyl) cobalt(III)] perchlorate liberates molecular oxygen upon irradiation with near-UV light. A mixture of cells and the caged oxygen compound was placed in a capillary tube and challenged by discrete stimuli of molecular oxygen produced by photolysis. We then recorded the rate of change of direction (rcd) as an estimate of tumble frequency in response to liberated oxygen and measured the signal processing (excitation) times in Bacillus subtilis, Bacillus halodurans and Escherichia coli. This computer-assisted caged oxygen assay gives a unique physiological profile of different aerotaxis transducers in bacteria.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2002.tb11481.x