Estrogen regulates GFAP-expression in specific subnuclei of the female rat interpeduncular nucleus: a potential role for estrogen receptor β

• Published in: Brain research 2002-12, Vol.958 (2), p.488-496
• Main Authors: Zsarnovszky, Attila, Smith, Todd, Hajos, Ferenc, Belcher, Scott M.
• Format: Article
• Language: English
• Subjects: Animals; Astrocyte; Biochemistry and metabolism; Biological and medical sciences; Central nervous system; Estradiol - pharmacology; Estrogen; Estrogen Receptor beta; Estrogens - pharmacology; Female; Fundamental and applied biological sciences. Psychology; Glia, glial fibrillary acidic protein; Glial Fibrillary Acidic Protein - analysis; Glial Fibrillary Acidic Protein - biosynthesis; Immunohistochemistry; Interpeduncular nucleus; Mesencephalon - chemistry; Mesencephalon - drug effects; Mesencephalon - metabolism; Ovariectomy; Rats; Rats, Sprague-Dawley; Receptors, Estrogen - analysis; Receptors, Estrogen - physiology; Vertebrates: nervous system and sense organs
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/S0006-8993(02)03771-X

We previously demonstrated that in rat, astrocytic glial fibrillary acidic protein- (GFAP) expression in the interpeduncular nucleus (IPN) was responsive to testosterone and in females the intensity of GFAP-immunoreactivity (IR) followed the periodic hormonal changes of the estrous cycle. The aim of this study was to test whether 17β-estradiol (E 2), in the absence of other ovarian hormones, can influence GFAP-expression within individual subnuclei of the IPN and to determine the cellular distribution of estrogen receptor β (ERβ) in the IPN. Quantitative surface-density analysis was used to compare the intensity of GFAP-IR at different anterio-posterior (AP) levels of the IPN in ovariectomized female rats 24 h after treatment with E 2 or vehicle. Estrogen-treatment resulted in a significant increase in GFAP-IR in the rostrolateral subnucleus of the IPN at AP: −5.60, in the lateral-, dorsolateral-, dorsomedial- and central subnuclei at −6.04 and in the lateral subnucleus at −6.72. No significant differences were observed at −5.80 and −6.30. These results indicate that E 2, in the absence of other ovarian hormones, modulates GFAP-expression within select IPN subnuclei and that these affects are dependent on position along the AP axis. To determine whether ERβ was a possible mediator of the observed estrogenic effects, adjacent section pairs of the IPN were immunostained for ERβ or GFAP. Using the ‘mirror’ method, ERβ-IR was detected in the cytoplasm of GFAP-immunopositive astroglia and in the nuclei of GFAP-immunonegative neurons. These findings suggest that in the IPN, E 2 may directly modulate GFAP-expression through ERβ-mediated mechanisms.