Human bone marrow angiogenesis: in vitro modulation by substance P and neurokinin A

• Published in: British journal of haematology 2002-12, Vol.119 (4), p.1083-1089
• Main Authors: Pelletier, Laurent, Angonin, Regis, Regnard, Jacques, Fellmann, Dominique, Charbord, Pierre
• Format: Article
• Language: English
• Subjects: angiogenesis; Angiogenesis Inducing Agents - pharmacology; Biological and medical sciences; Bone Marrow - blood supply; Bone Marrow - innervation; Bone Marrow Cells - drug effects; Cell Culture Techniques - methods; Dose-Response Relationship, Drug; Fundamental and applied biological sciences. Psychology; human bone marrow; Humans; Immunoenzyme Techniques; innervation; Microcirculation; microenvironment; Neovascularization, Physiologic - drug effects; Nerve Fibers - ultrastructure; Neurokinin A - pharmacology; Substance P - pharmacology; tachykinins; Vertebrates: blood, hematopoietic organs, reticuloendothelial system
• ISSN: 0007-1048; 1365-2141
• DOI: 10.1046/j.1365-2141.2002.03969.x

We have previously described a culture system for human bone marrow endothelial cells that organize into capillary tubes associated to pericytes. In the present work, we used this model to assess the angiogenic properties of tachykinins, which have been demonstrated to be involved in neuro–immuno–haematopoietic interactions. The substance P (SP) and neurokinin A (NKA) were similarly potent at increasing in vitro angiogenesis, via NK1 and NK2 receptors respectively. These mediators were not produced by cells in culture, suggesting that in vivo they may be released by nerve fibres in the bone marrow. Therefore, we looked for in situ innervation of the human bone marrow, unknown to date, using immunohistochemistry techniques. As in rodents, arterioles were largely innervated, associated with between one and 10 nerve fibres. Capillary innervation was more restrictive as a unique thin nerve fibre was found in the vicinity of only 6% of these vessels. Finally, no nerve fibres were observed in the vicinity of sinus walls. In conclusion, both in vitro results and the anatomical display of nerve fibres suggest a role in human bone marrow for the vasoactive neuropeptides SP and NKA, which were secreted into a perivascular location. These neural mediators might modulate blood flow in the bone marrow both in the short term by adjusting vascular tone and in the long term by inducing angiogenesis.