Introduction of a Na+/H+ antiporter gene from Atriplex gmelini confers salt tolerance to rice

We engineered a salt-sensitive rice cultivar (Oryza sativa cv. Kinuhikari) to express a vacuolar-type Na+/H+ antiporter gene from a halophytic plant, Atriplex gmelini (AgNHX1). The activity of the vacuolar-type Na+/H+ antiporter in the transgenic rice plants was eight-fold higher than that in wild-t...

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Bibliographic Details
Published in:FEBS letters 2002-12, Vol.532 (3), p.279-282
Main Authors: Ohta, Masaru, Hayashi, Yasuyuki, Nakashima, Asae, Hamada, Akira, Tanaka, Akira, Nakamura, Tatsunosuke, Hayakawa, Takahiko
Format: Article
Language:English
Subjects:
Caryophyllaceae - genetics
Caryophyllaceae - metabolism
Dose-Response Relationship, Drug
Halophyte plants
Oryza - genetics
Oryza - metabolism
Plants, Genetically Modified
Rice
RNA - metabolism
Salt tolerance
Salts - pharmacology
Sodium - pharmacology
Sodium-Hydrogen Exchangers - genetics
Sodium-Hydrogen Exchangers - physiology
Sodium/proton antiporter
Spectrometry, Fluorescence
Time Factors
Transformation, Genetic
Transgenic plants
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Summary:We engineered a salt-sensitive rice cultivar (Oryza sativa cv. Kinuhikari) to express a vacuolar-type Na+/H+ antiporter gene from a halophytic plant, Atriplex gmelini (AgNHX1). The activity of the vacuolar-type Na+/H+ antiporter in the transgenic rice plants was eight-fold higher than that in wild-type rice plants. Salt tolerance assays followed by non-stress treatments showed that the transgenic plants overexpressing AgNHX1 could survive under conditions of 300 mM NaCl for 3 days while the wild-type rice plants could not. These results indicate that overexpression of the Na+/H+ antiporter gene in rice plants significantly improves their salt tolerance.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(02)03679-7