A Pectin Glucuronyltransferase Gene Is Essential for Intercellular Attachment in the Plant Meristem

Intercellular attachment is an essential process in the morphogenesis of multicellular organisms. A unique mutant, nolac-H18 (non-organogenic callus with loosely attached cells), generated by T-DNA transformation using leaf-disk cultures of haploid Nicotiana plumbaginifolia, lost the ability to form...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2002-12, Vol.99 (25), p.16319-16324
Main Authors: Iwai, Hiroaki, Masaoka, Nobutaka, Ishii, Tadashi, Satoh, Shinobu
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Biological Sciences
Borates
Botany
Callus
Carbohydrate Sequence
Carbohydrates - analysis
Cell Adhesion
Cell Wall - chemistry
Cell walls
Cellular biology
Dimerization
Dimers
Genes
Genes, Recessive
Genetic mutation
Glucuronosyltransferase - genetics
Glucuronosyltransferase - physiology
Haploidy
Meristem - cytology
Meristem - metabolism
Meristems
Microscopy, Electron, Scanning
Molecular Sequence Data
Mutation
Nicotiana - enzymology
Pectins - biosynthesis
Phenotypes
Plant cells
Plant Proteins - genetics
Plant Proteins - physiology
Plant Shoots
Plants
RNA, Antisense - pharmacology
RNA, Messenger - biosynthesis
RNA, Plant - biosynthesis
Sequence Alignment
Sequence Homology, Amino Acid
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Summary:Intercellular attachment is an essential process in the morphogenesis of multicellular organisms. A unique mutant, nolac-H18 (non-organogenic callus with loosely attached cells), generated by T-DNA transformation using leaf-disk cultures of haploid Nicotiana plumbaginifolia, lost the ability to form tight intercellular attachments and adventitious shoots. The gene tagged with T-DNA, named NpGUT1 (glucuronyltransferase 1), was similar to the gene for the catalytic domains of animal glucuronyltransferases and was expressed predominantly in shoot and root apical meristems. The transformation of NpGUT1 complemented the nolac-H18 mutation, and the expression of antisense NpGUT1 RNA produced crumbled shoots. The mutation caused defects in the glucuronic acid of rhamnogalacturonan II of pectin, which drastically reduced the formation of borate cross-linking of rhamnogalacturonan II. NpGUT1, which encodes a unique glucuronyltransferase, is a glycosyltransferase gene identified in pectin biosynthesis and is essential for intercellular attachment in plant meristems and tissues.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.252530499