Cytokine production in response to endodontic infection in germ-free mice

This study evaluated the cytokine profiles (type 1 or type 2) that are triggered by and modulate endodontic periapical infections in the root canal system of germ‐free mice. Microorganisms isolated from two patients with pulpal necrosis were inoculated into two groups of experimental animals: group...

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Bibliographic Details
Published in:Oral microbiology and immunology 2002-12, Vol.17 (6), p.344-353
Main Authors: Ribeiro Sobrinho, A. P., De Melo Maltos, S. M., Farias, L. M., De Carvalho, M. A. R., Nicoli, J. R., De Uzeda, M., Vieira, L. Q.
Format: Article
Language:English
Subjects:
Animals
Bacterial diseases
Bifidobacteriales Infections - immunology
Bifidobacterium - immunology
Biological and medical sciences
Clostridium Infections - immunology
cytokines
Cytokines - biosynthesis
Dental Pulp Cavity - microbiology
Dental Pulp Necrosis - microbiology
Dentistry
Down-Regulation
endodontic infections
Enzyme-Linked Immunosorbent Assay
Experimental bacterial diseases and models
Fusobacterium Infections - immunology
Fusobacterium nucleatum - immunology
Germ-Free Life
germ-free mice
Gram-Positive Bacterial Infections - immunology
Humans
Infectious diseases
Interferon-gamma - biosynthesis
Interleukin-12 - biosynthesis
Interleukin-4 - biosynthesis
Lymph Nodes - immunology
Lymph Nodes - pathology
Medical sciences
Mice
microorganisms
pathogenesis
Periapical Diseases - immunology
Periapical Diseases - microbiology
Spleen - immunology
Spleen - pathology
Staphylococcaceae - immunology
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Summary:This study evaluated the cytokine profiles (type 1 or type 2) that are triggered by and modulate endodontic periapical infections in the root canal system of germ‐free mice. Microorganisms isolated from two patients with pulpal necrosis were inoculated into two groups of experimental animals: group I (Gemella morbillorum) and group II (Bifidobacterium adolescentis, Fusobacterium nucleatum and Clostridium butyricum). In vitro, G. morbillorum induced type 1 cytokine synthesis, while the modulation processed in vivo seemed to have the opposite effect, with a reduction in the basal levels of IL‐12 and IFN‐γ, IL‐4‐independent down‐modulation. In vitro, microorganisms from group II, in poly‐infection, induced a reduction of type 1 cytokine levels from day 10 to day 20, which seemed to be modulated via IL‐4. In vivo, however, a predominance of the immune response to one species over the others occurred.
ISSN:0902-0055
1399-302X
DOI:10.1034/j.1399-302X.2002.170603.x