Effect of Andrographolide and Kan Jang – fixed combination of extract SHA-10 and extract SHE-3 – on proliferation of human lymphocytes, production of cytokines and immune activation markers in the whole blood cells culture

• Published in: Phytomedicine (Stuttgart) 2002-10, Vol.9 (7), p.598-605
• Main Authors: Panossian, A., Davtyan, T., Gukassyan, N., Gukasova, G., Mamikonyan, G., Gabrielian, E., Wikman, G.
• Format: Article
• Language: English
• Subjects: Andrographis; Andrographis paniculata; andrographolide; Biomarkers; Blood Cells - cytology; Blood Cells - drug effects; Blood Cells - metabolism; Cells, Cultured; Cytokines - biosynthesis; Diterpenes - pharmacology; Eleutherococcus; Eleutherococcus senticosus; Glucosides - pharmacology; Humans; INF-γ; Interferon-gamma - biosynthesis; Interferon-gamma - drug effects; Kan Jang; Lignans; lymphocytes; Lymphocytes - cytology; Lymphocytes - drug effects; Lymphocytes - metabolism; neopterin; Plant Extracts - pharmacology; SHA-10; sIL-2R; TNF-α; Tumor Necrosis Factor-alpha - biosynthesis; β-2-microglobulin
• ISSN: 0944-7113; 1618-095X
• DOI: 10.1078/094471102321616409

The immunomodulatory properties of a diterpene lactone andrographolide and Kan Jang – a standardized fixed combination of Andrographis paniculata extract SHA-10 and Eleutherococcus senticosus extract SHE-3 were investigated. Their role on spontaneous and phytohemagglutinin (PHA)-induced proliferation of human peripheral blood lymphocytes (PBL) and on production of interferon-gamma (INF-γ) and tumor necrosis factor-alpha (TNF-α) were determined in vitro. Proliferation of PBL induced by PHA was enhanced by co stimulation with andrographolide and Kan Jang. At the same time andrographolide and Kan Jang inhibit spontaneous proliferation of PBL in vitro. These preparations also have effect on the formation of INF-γ, TNF-α and some immune activation markers such as neopterin (Neo), β-2-microglobulin (β2MG), and soluble receptor for interleukin-2 (sIL-2R or sCD25) in blood cells culture. Andrographolide and Kan Jang stimulate the INF-γ, Neopterin and β2MG formation, but do not have any significant effect on the production of INF-γ and Neopterin in PHA stimulated blood cells. An opposite effect on these immune makers was observed in the PHA-stimulated blood cells: both andrographolide and Kan Jang increase the formation of TNF-α and β2MG in cultivated whole blood cells. Thus, andrographolide and Kan Jang can have an in vitro effect on the activation and proliferation of immunocompetent cells as well on the production of key cytokines and immune activation markers. The results show an overall higher effect of the fixed combination as compared with the equivalent amount of the pure substance andrographolide. The data are consistent with results from clinical studies of Kan Jang and contributed to a better understanding of these results.