Immunovisualization of High (HK) and Low (LK) Molecular Weight Kininogens on Isolated Human Neutrophils

An immunocytochemical study was performed to examine the cellular localization and the subcellular distribution of kininogens in human blood cells. Kininogens were visualised using the immunogold-silver staining method and confocal scanning laser microscopy. We confirmed the existence of high molecu...

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Bibliographic Details
Published in:Blood 1992-02, Vol.79 (3), p.754-759
Main Authors: Figueroa, Carlos D., Henderson, Lydia M., Kaufmann, Jorg, De La Cadena, Raul A., Colman, Robert W., Müller-Esterl, Werner, Bhoola, Kanti D.
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal
Biological and medical sciences
Blood Platelets - enzymology
Cell Membrane - enzymology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunobiology
Immunohistochemistry
In Vitro Techniques
Kininogens - chemistry
Kininogens - metabolism
Molecular Weight
Myeloid cells: ontogeny, maturation, markers, receptors
Neutrophils - enzymology
Polynuclears
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Summary:An immunocytochemical study was performed to examine the cellular localization and the subcellular distribution of kininogens in human blood cells. Kininogens were visualised using the immunogold-silver staining method and confocal scanning laser microscopy. We confirmed the existence of high molecular weight kininogen in human neutrophils and describe for the first time the presence of low molecular weight kininogen on these cells. Both high and low molecular weight kininogens were restricted to the neutrophils where they localized as clusters of immunogold particles on the cell membrane. No labeling was observed intracellularly in organelles such as mitochondria, endoplasmic reticulum, and azurophilic or specific granules after permeabilization of the neutrophils with Triton X-100, a procedure that permitted the visualization of elastase in the azurophilic granules. Clusters of high molecular weight kininogen molecules attached to the neutrophil surface could serve as receptors for plasma kallikrein and/or be the source of substrate for a discrete and circumscribed formation of kinins that may in turn facilitate the local diapedesis of neutrophils and the transudation of plasma constituents during acute inflammation.© 1992 by The American Society of Hematology. 0006–4971/92/7903-0027$3.00/0
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V79.3.754.754