Loss of Heterozygosity of p53, BRCA1, VHL, and Estrogen Receptor Genes in Breast Carcinoma: Correlation with Related Protein Products and Morphologic Features

Correlation of loss of heterozygosity (LOH) of p53, BRCA1, VHL, and estrogen receptor (ER) genes with the expression of related protein products and morphologic features predictive of aggressive biologic behavior was investigated to determine the significance of LOH in these genes. DNA from 35 forma...

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Bibliographic Details
Published in:International journal of surgical pathology 2002-10, Vol.10 (4), p.237-245
Main Authors: Otis, Christopher N., Krebs, Patricia A., Albuquerque, Ana, Quezado, Martha M., Juan, Xavier San, Sobel, Mark E., Merino, Maria J.
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - pathology
Adult
Aged
Aged, 80 and over
Biomarkers, Tumor - metabolism
BRCA1 Protein - genetics
BRCA1 Protein - metabolism
Breast Neoplasms - genetics
Breast Neoplasms - pathology
DNA, Neoplasm - analysis
Female
Genes, BRCA1
Genes, p53 - genetics
Humans
Immunohistochemistry
Ligases - genetics
Ligases - metabolism
Loss of Heterozygosity
Microsatellite Repeats - genetics
Middle Aged
Polymerase Chain Reaction
Receptors, Estrogen - genetics
Receptors, Estrogen - metabolism
Tumor Suppressor Protein p53 - genetics
Tumor Suppressor Protein p53 - metabolism
Tumor Suppressor Proteins
Ubiquitin-Protein Ligases
Von Hippel-Lindau Tumor Suppressor Protein
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Summary:Correlation of loss of heterozygosity (LOH) of p53, BRCA1, VHL, and estrogen receptor (ER) genes with the expression of related protein products and morphologic features predictive of aggressive biologic behavior was investigated to determine the significance of LOH in these genes. DNA from 35 formalin-fixed, paraffin-embedded breast carcinomas was obtained by microdissection of histologic sections. LOH was determined by polymerase chain reaction (PCR) using primers TP53, D3S1038, D17S855, and ESR for p53, VHL, BRCA1, and ER genes, respectively. p53, ER, and progesterone receptor (PR) protein expression was evaluated by immunohistochemistry. Morphologic evaluation included histologic type, and histologic and nuclear grades. TP53 LOH was identified in 13 (52%), BRCA1 LOH in 3 (17%), VHL LOH in 1 (4%), and ER LOH in 4 (21%) of 25, 17, 24, and 19 informative cases, respectively. p53 and ER protein expression was identified in 20 (57%) and 25 (71%) cases, respectively. TP53 LOH directly correlated with both high histologic and nuclear grade (P
ISSN:1066-8969
1940-2465
DOI:10.1177/106689690201000401