Segregation of transgenes in maize

Progeny recovered from backcrossed transgenic maize tissue culture regenerants (R0) were analyzed to determine the segregation, expression, and stability of the introduced genes. Transgenic A188 x B73 R0 plants (regenerated from embryogenic suspension culture cells transformed by microprojectile bom...

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Bibliographic Details
Published in:Plant molecular biology 1992, Vol.18 (2), p.201-210
Main Authors: Spencer, T.M. (DEKALB Plant Genetics, Groton, CT (USA). Discovery Research), O'Brien, J.V, Start, W.G, Adams, T.R, Gordon-Kamm, W.J, Lemaux, P.G
Format: Article
Language:English
Subjects:
Acetyltransferases - genetics
Acetyltransferases - metabolism
Aminobutyrates - pharmacology
Blotting, Southern
DESCENDANCE
Drug Resistance - genetics
GENE
Gene Expression
GENES
GENETIC INHERITANCE
Genetic Linkage - genetics
Genetic Markers - genetics
GENETIC TRANSFORMATION
Genetic Vectors - genetics
HEREDITE
HERENCIA GENETICA
inheritance
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants, Genetically Modified - drug effects
Plants, Genetically Modified - enzymology
Plants, Genetically Modified - genetics
Plasmids - genetics
PROGENIE
PROGENY
SEGREGACION
SEGREGATION
TRANSFORMACION GENETICA
transformation
TRANSFORMATION GENETIQUE
Transformation, Genetic
TRANSGENIC PLANTS
ZEA MAYS
Zea mays - drug effects
Zea mays - enzymology
Zea mays - genetics
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Summary:Progeny recovered from backcrossed transgenic maize tissue culture regenerants (R0) were analyzed to determine the segregation, expression, and stability of the introduced genes. Transgenic A188 x B73 R0 plants (regenerated from embryogenic suspension culture cells transformed by microprojectile bombardment; see [9]) were pollinated with nontransformed B73 pollen. Inheritance of a selectable marker gene, bar, and a nonselectable marker gene, uidA, was analyzed in progeny (R1) representing four independent transformation events. Activity of the bar gene product, phosphinothricin acetyltransferase (PAT), was assessed in plants comprising the four R1 populations. The number of R1 plants containing PAT activity per total number of R1 plants recovered for each population was 2/7, 19/34, 3/14 and 73/73. Molecular analysis confirmed the segregation of bar in three R1 populations and the lack of segregation in one R1 population. Cosegregation analysis indicated genetic linkage of bar and uidA in all four R1 populations. Analysis of numerous R2 plants derived from crossing transformed R1 plants with nontransformed inbreds revealed 1:1 segregation of PAT activity in three of four lines, including the line that failed to segregate in the R1 generation. Integrated copies of bar in one line appeared to be unstable or poorly transmitted.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00034949