Detection of Knockdown Resistance Mutations in Anopheles sacharovi (Diptera: Culicidae) and Genetic Distance with Anopheles gambiae (Diptera: Culicidae) Using cDNA Sequencing of the Voltage-Gated Sodium Channel Gene

The knockdown resistance (kdr) mutation in the voltage-gated sodium channel gene (VGSCG), an important resistance mechanism against pyrethroids, was studied in Anopheles sacharovi Favre. It was found that the specific primers Agdl and Agd2 used for polymerase chain reaction (PCR) amplification of An...

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Bibliographic Details
Published in:Journal of medical entomology 2002-11, Vol.39 (6), p.870-874
Main Authors: Lüleyap, HÜmit, Alptekin, Davut, Kasap, Halil, Kasap, Mulkiye
Format: Article
Language:English
Subjects:
Animals
Anopheles - classification
Anopheles - genetics
Anopheles - physiology
Anopheles gambiae
Anopheles sacharovi
Base Sequence
Biological and medical sciences
DNA Primers
DNA, Complementary
Fundamental and applied biological sciences. Psychology
genetic distance
Genetics of eukaryotes. Biological and molecular evolution
Immunity, Innate
Invertebrata
Knockdown resistance mutation
Medically important nuisances and vectors, pests of stored products and materials: population survey and control
MOLECULAR BIOLOGY/GENOMICS
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Population genetics, reproduction patterns
Sodium Channels - genetics
Vectors. Intermediate hosts
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Summary:The knockdown resistance (kdr) mutation in the voltage-gated sodium channel gene (VGSCG), an important resistance mechanism against pyrethroids, was studied in Anopheles sacharovi Favre. It was found that the specific primers Agdl and Agd2 used for polymerase chain reaction (PCR) amplification of Anopheles gambiae Giles VGSCG also amplified this genomic region in An. sacharovi. Comparison of the IIs4–IIs6 domain segments of the gene indicated 70% nucleotides common to both species and a genetic distance of 0.255 between them. Four different samples of pyrethroid-resistant An. sacharovi produced three types of amino acid, serine (TCG), leucine (TTG), and phenylalanine (TTT) at the kdr mutation point, whereas only two kdr mutations, leucine to phenylalanine and leucine to serine, occur in An. gambiae.
ISSN:0022-2585
1938-2928
DOI:10.1603/0022-2585-39.6.870