The use of laser microdissection for the preparation of chromosome-specific painting probes in farm animals

Laser microbeam microdissection and laser pressure catapulting procedure were used for the construction of chromosome-specific painting probes, arm-specific probes and probes for chromosomal subfragments. We report on a method for generation of fluorescence in-situ hybridization probes from laser di...

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Bibliographic Details
Published in:Chromosome research 2002-01, Vol.10 (7), p.571-577
Main Authors: Kubickova, Svatava, Cernohorska, Halina, Musilova, Petra, Rubes, Jiri
Format: Article
Language:English
Subjects:
Animals
Animals, Domestic - genetics
Cattle
Chromosome Banding
Chromosome Painting - methods
Chromosomes
Chromosomes - genetics
Dissection - methods
DNA Probes
Equidae
Genetic Markers
Heterozygote
In Situ Hybridization, Fluorescence
Karyotyping
Lasers
Species Specificity
Swine
Taq Polymerase - genetics
Translocation, Genetic
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Summary:Laser microbeam microdissection and laser pressure catapulting procedure were used for the construction of chromosome-specific painting probes, arm-specific probes and probes for chromosomal subfragments. We report on a method for generation of fluorescence in-situ hybridization probes from laser dissected chromosomes of farm animals. So far, using the described method, a set of chromosome-specific painting probes has been obtained for all porcine chromosomes, 17 chromosomes of cattle and selected equine chromosomes. It is concluded that the laser technology appears to be a useful and powerful tool for the construction of chromosome-specifi c painting probes. Its main advantage is the fast non-contact collection of chromosomes.
ISSN:0967-3849
1573-6849
DOI:10.1023/a:1020914702767