Plastid DNA from Pyrenomonas salina (Cryptophyceae): physical map, genes, and evolutionary implications

Cryptomonads are thought to have arisen from a symbiotic association between a eukaryotic flagellated host and a eukaryotic algal symbiont, presumably related to red algae. As organellar DNAs have proven to be useful tools in elucidating phylogenetic relationships, the plastid (pt) DNA of the crypto...

Full description

Saved in:
Bibliographic Details
Published in:Current genetics 1992, Vol.21 (1), p.73-81
Main Authors: Maerz, M. (Institut fuer Biologie II, Freiburg (Germany). Lehrstuhl fuer Zellbiologie), Wolters, J, Hofmann, C.J.B, Sitte, P, Maier, U.G
Format: Article
Language:English
Subjects:
ADN
ALGAE
ARN
Base Sequence
Biological and medical sciences
Biological Evolution
Brackish
CARTE GENETIQUE
Classical genetics, quantitative genetics, hybrids
Cloning, Molecular
Cryptomonads
Cryptophyta
DNA
DNA, Circular - chemistry
DNA, Circular - genetics
DNA, Circular - isolation & purification
Eukaryota - classification
Eukaryota - genetics
EVOLUCION
EVOLUTION
Freshwater
Fundamental and applied biological sciences. Psychology
GENE
GENES
GENETIC MAPS
Genetics of eukaryotes. Biological and molecular evolution
MAPAS GENETICOS
Marine
Molecular Sequence Data
NUCLEOTIDE SEQUENCE
Operon
Organelles - chemistry
Phylogeny
PLASTE
Plastid DNA
Plastid evolution
PLASTIDIOS
PLASTIDS
Pyrenomonas salina
Restriction Mapping
Rhodophyta
RNA
RNA, Ribosomal - genetics
SECUENCIA NUCLEICA
Sequence Alignment
SEQUENCE NUCLEIQUE
SSUrRNA sequence
Thallophyta, bryophyta
Vegetals
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cryptomonads are thought to have arisen from a symbiotic association between a eukaryotic flagellated host and a eukaryotic algal symbiont, presumably related to red algae. As organellar DNAs have proven to be useful tools in elucidating phylogenetic relationships, the plastid (pt) DNA of the cryptomonad alga Pyrenomonas salina has been characterized in some detail. A restriction map of the circular 127 kb ptDNA from Pyrenomonas salina was established. An inverted repeat (IR) region of about 5 kb separates two single-copy regions of 15 and 102 kb, respectively. It contains the genes for the small and large subunit of rRNA. Ten protein genes, coding for the large subunit of ribulose-1,5-bisphosphate carboxylase, the 47 kDa, 43 kDa and 32 kDa proteins of photosystem II, the ribosomal proteins L2, S7 and S11, the elongation factor Tu, as well as the alpha- and beta-subunits of ATP synthase, have been localized on the restriction map either by hybridization of heterologous gene probes or by sequence homologies. The gene for the plastidal small subunit (SSUr) RNA has been sequenced and compared to homologous SSU regions from the cyanobacterium Anacystis nidulans and plastids from rhodophytes, chromophytes, euglenoids, chlorophytes, and land plants. A phylogenetic tree constructed with the neighborliness method and indicating a relationship of cryptomonad plastids with those of red algae is presented.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00318658