Cellular platforms for HTS: three case studies

The field of cell-based screening is expanding rapidly as innovations in target selection and instrumentation increase the number of targets that can be efficiently screened in cellular formats. Cell-based screens can be configured to provide a broad range of data on chemical compound activity, mech...

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Bibliographic Details
Published in:Drug Discovery Today 2002-03, Vol.7 (6), p.353-363
Main Authors: Johnston, Paul A, Johnston, Patricia A
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cell-based assays
Cells, Cultured
Cytological Techniques
Drug Evaluation, Preclinical - economics
Drug Evaluation, Preclinical - trends
General pharmacology
GPCR's, transporters lead generation
high-throughput screens
Humans
Luciferases - genetics
Medical sciences
Pharmaceutical technology. Pharmaceutical industry
Pharmacology - trends
Pharmacology. Drug treatments
Radiopharmaceuticals - chemistry
reporters
Reproducibility of Results
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Summary:The field of cell-based screening is expanding rapidly as innovations in target selection and instrumentation increase the number of targets that can be efficiently screened in cellular formats. Cell-based screens can be configured to provide a broad range of data on chemical compound activity, mechanism of action and drugability. However, the decision to pursue a cell-based approach should not be made lightly, as cell-based assays can be challenging to implement in the high-throughput screening (HTS) laboratory. In this review, we describe three case studies in which targets were successfully interrogated in cell-based HTS, and highlight the necessary steps to ensure the validity of these screens.
ISSN:1359-6446
1878-5832
DOI:10.1016/S1359-6446(01)02140-7