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Cellular platforms for HTS: three case studies

The field of cell-based screening is expanding rapidly as innovations in target selection and instrumentation increase the number of targets that can be efficiently screened in cellular formats. Cell-based screens can be configured to provide a broad range of data on chemical compound activity, mech...

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Published in:	Drug Discovery Today 2002-03, Vol.7 (6), p.353-363
Main Authors:	Johnston, Paul A, Johnston, Patricia A
Format:	Article
Language:	English
Subjects:	Animals Biological and medical sciences Carrier Proteins - genetics Carrier Proteins - metabolism Cell-based assays Cells, Cultured Cytological Techniques Drug Evaluation, Preclinical - economics Drug Evaluation, Preclinical - trends General pharmacology GPCR's, transporters lead generation high-throughput screens Humans Luciferases - genetics Medical sciences Pharmaceutical technology. Pharmaceutical industry Pharmacology - trends Pharmacology. Drug treatments Radiopharmaceuticals - chemistry reporters Reproducibility of Results
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description	The field of cell-based screening is expanding rapidly as innovations in target selection and instrumentation increase the number of targets that can be efficiently screened in cellular formats. Cell-based screens can be configured to provide a broad range of data on chemical compound activity, mechanism of action and drugability. However, the decision to pursue a cell-based approach should not be made lightly, as cell-based assays can be challenging to implement in the high-throughput screening (HTS) laboratory. In this review, we describe three case studies in which targets were successfully interrogated in cell-based HTS, and highlight the necessary steps to ensure the validity of these screens.
doi_str_mv	10.1016/S1359-6446(01)02140-7
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source	ScienceDirect Journals
subjects	Animals Biological and medical sciences Carrier Proteins - genetics Carrier Proteins - metabolism Cell-based assays Cells, Cultured Cytological Techniques Drug Evaluation, Preclinical - economics Drug Evaluation, Preclinical - trends General pharmacology GPCR's, transporters lead generation high-throughput screens Humans Luciferases - genetics Medical sciences Pharmaceutical technology. Pharmaceutical industry Pharmacology - trends Pharmacology. Drug treatments Radiopharmaceuticals - chemistry reporters Reproducibility of Results
title	Cellular platforms for HTS: three case studies
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