An Example of Neural Plasticity Evoked by Putative Behavioral Demand and Early Use of Vibrissal Hairs after Facial Nerve Transection

• Published in: Experimental neurology 2002-12, Vol.178 (2), p.207-218
• Main Authors: Tomov, Toma L., Guntinas-Lichius, Orlando, Grosheva, Maria, Streppel, Michael, Schraermeyer, Ulrich, Neiss, Wolfram F., Angelov, Doychin N.
• Format: Article
• Language: English
• Subjects: Animals; Behavior, Animal - physiology; Biological and medical sciences; Blindness - pathology; Blindness - physiopathology; Development. Senescence. Regeneration. Transplantation; Facial Nerve Injuries - pathology; Facial Nerve Injuries - physiopathology; Female; Fundamental and applied biological sciences. Psychology; Motor Activity - physiology; Neuronal Plasticity - physiology; Rats; Rats, Sprague-Dawley; Vertebrates: nervous system and sense organs; Vibrissae - physiology; Video Recording - methods
• ISSN: 0014-4886; 1090-2430
• DOI: 10.1006/exnr.2002.8040

Abnormally associated movements inevitably occur after surgical repair of the facial nerve. The reason for this postparalytic syndrome is poor navigation of regrowing axons. Despite the valuable functional advantage provided by the easily detected movement of vibrissae in rats, the major investigative tools for establishing the degree of misdirected reinnervation are still electrophysiologic recordings and retrograde tracing. In the present study we complemented data from pre- and postoperative retrograde labeling (FluoroGold, Fast Blue, DiI) of facial motoneurons with an evaluation of whisker movements. Using a video-based motion analysis system, we compared the recovery of vibrissae motor performance in visually normal and blind rats of the Sprague–Dawley strain. The analysis of whisker movement after facial nerve surgery revealed a striking discrepancy between morphologic and functional estimates. Whereas retrograde labeling displayed poor accuracy of target reinnervation and supernumerary axonal branching in both groups, the video-based motion analysis showed a perfect recovery of vibrissae movements in the blind rats. Attributing the complete recovery of whisker movement in the blind rats to an extraordinary plasticity of the facial motoneurons induced by putative behavioral demand and forced overuse, we conclude that the video-based analysis of whisker movement is a valuable tool for studying the progress in functional recovery.