Identification of Dirofilaria immitis-Infected Dogs with a Coagglutination Assay

The presence of Dirofilaria immitis excretory-secretory (ES) products was detected in the urine of infected dogs using a coagglutination assay. Urine samples from 30 naturally infected dogs were positive. Seventeen of them were microfilaremic, whereas 13 had become amicrofilaremic after receiving 2...

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Bibliographic Details
Published in:The Journal of parasitology 1992-02, Vol.78 (1), p.166-168
Main Authors: Rojas, Lázara, Duménigo, Blanca E., Fonte, Luis, Fachado, Alberto, Finlay, Carlos M.
Format: Article
Language:English
Subjects:
Agglutination Tests - veterinary
Animals
Antigens
Antigens, Helminth - urine
Biological and medical sciences
Canines
Chemical suspensions
Diethylcarbamazine - therapeutic use
Dirofilaria immitis - immunology
Dirofilaria immitis - isolation & purification
Dirofilariasis - diagnosis
Dirofilariasis - drug therapy
Dirofilariasis - parasitology
Dirofilariasis - veterinary
Dog Diseases - diagnosis
Dog Diseases - drug therapy
Dog Diseases - parasitology
Dogs
Filariasis
Fundamental and applied biological sciences. Psychology
General aspects and techniques. Study of several systematic groups. Models
Infections
Invertebrates
Irish culture
Miscellaneous techniques
Parasites
Parasitology
Research Notes
Sensitivity and Specificity
Techniques
Urine
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Summary:The presence of Dirofilaria immitis excretory-secretory (ES) products was detected in the urine of infected dogs using a coagglutination assay. Urine samples from 30 naturally infected dogs were positive. Seventeen of them were microfilaremic, whereas 13 had become amicrofilaremic after receiving 2 courses of diethylcarbamazine. Urine samples from 20 dogs infected with other parasites, Dipetalonema reconditum (7), Toxocara cards (5), and Ancylostoma caninum (8), and urine samples from 20 healthy dogs were negative. The assay detected 200 ng/ml or more of ES products. This assay is simple, easy to perform with minimum training, and requires no equipment. Therefore it should be useful to detect canine filariasis under field conditions.
ISSN:0022-3395
1937-2345
DOI:10.2307/3283711