Expression of inducible nitric oxide synthase in the lower esophageal sphincter of the endotoxemic opossum

Endotoxin modulates esophageal motor function by increasing nitric oxide (NO) production. The aims of this study were to examine inducible nitric oxide synthase (iNOS) induction in the lower esophageal sphincter (LES) of endotoxemic opossums and to investigate the effects of aminoguanidine (AG), a s...

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Bibliographic Details
Published in:Journal of gastroenterology 2002-12, Vol.37 (12), p.1000-1004
Main Authors: Park, Hyojin, Clark, Eugene, Cullen, Joseph J, Koland, John G, Kim, Myong Soo, Conklin, Jeffrey L
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Blotting, Western
Disease Models, Animal
Endotoxemia - enzymology
Endotoxemia - physiopathology
Esophagogastric Junction - drug effects
Esophagogastric Junction - physiopathology
Female
Gene Expression Regulation
Guanidines - pharmacology
Lipopolysaccharides
Male
Molecular Sequence Data
Nitric Oxide - analysis
Nitric Oxide - metabolism
Nitric Oxide Synthase - genetics
Nitric Oxide Synthase - metabolism
Nitric Oxide Synthase Type II
Opossums
Probability
Random Allocation
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
Sensitivity and Specificity
Statistics, Nonparametric
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Summary:Endotoxin modulates esophageal motor function by increasing nitric oxide (NO) production. The aims of this study were to examine inducible nitric oxide synthase (iNOS) induction in the lower esophageal sphincter (LES) of endotoxemic opossums and to investigate the effects of aminoguanidine (AG), a selective inhibitor of iNOS, on plasma nitrite/nitrate levels and on iNOS protein and mRNA expression after exposure to lipopolysaccharide (LPS). Before and 12 h after the intravenous administration of LPS and/or AG, plasma nitrite/nitrate levels were determined. The iNOS protein and mRNA expression were investigated in the tissues taken from the LES by Western blot and reverse-transcriptase polymerase chain reaction (RT-PCR). Plasma nitrite/nitrate levels were significantly increased by LPS. The increase in plasma nitrite/nitrate produced by LPS was significantly decreased by AG. Western blot and RT-PCR demonstrated that iNOS expression was markedly increased by LPS, and attenuated slightly by AG. These studies support the hypothesis that endotoxin increases NO production by the induction of iNOS protein and mRNA.
ISSN:0944-1174
1435-5922
DOI:10.1007/s005350200169