Increase of xanthan production by cloning xps genes into wild-type Xanthomonas campestris

Previously, genomic banks of Xanthomonas campestris were constructed in Escherichia coli, using mobilizable broad‐host‐range cosmids as the vectors. Following conjugal transfer, genes involved in the biosynthesis of xanthan polysaccharide (XPS) were cloned by the ability to restore the mucoid phenot...

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Bibliographic Details
Published in:Letters in applied microbiology 1992-02, Vol.14 (2), p.43-46
Main Authors: Tseng, Y.H, Ting, W.Y, Chou, H.C, Yang, B.Y, Chen, C.C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
carbohydrate metabolism
Carbohydrate Sequence
cloning
Cloning, Molecular
Conjugation, Genetic
cosmids
Escherichia coli
Escherichia coli - genetics
Food Additives
food biotechnology
Fundamental and applied biological sciences. Psychology
gene expression
Gene Expression Regulation, Bacterial
gene transfer
genes
Genetic engineering
Genetic technics
genetic transformation
Methods. Procedures. Technologies
Modification of gene expression level
Molecular Sequence Data
Plasmids
Polysaccharides, Bacterial - biosynthesis
Polysaccharides, Bacterial - genetics
Restriction Mapping
wild strains
xanthan gum
Xanthomonas campestris
Xanthomonas campestris - genetics
Xanthomonas campestris - metabolism
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Summary:Previously, genomic banks of Xanthomonas campestris were constructed in Escherichia coli, using mobilizable broad‐host‐range cosmids as the vectors. Following conjugal transfer, genes involved in the biosynthesis of xanthan polysaccharide (XPS) were cloned by the ability to restore the mucoid phenotype to the non‐mucoid mutants. In this study, all clones were transferred into the wild‐type strain Xc17 to evaluate the effects of the cloned genes on XPS production. Most clones showed no significant effect; however, two plasmids, pP2401 and pP2201, caused 10 and 15% yield increases, respectively, compared with that of controls. While it was not clear how pP2201 caused the yield increase, the effect of pP2401 seemed to result from elevated phosphomannose isomerase activity. Since XPS synthesis in X. campestris is a very efficient process, only relatively small increases are to be expected; an enhancement of productivity by 10–15% is important to the commercial production of xanthan.
ISSN:0266-8254
1472-765X
DOI:10.1111/j.1472-765X.1992.tb00643.x