Determination of chromium in cerebrospinal fluid using electrothermal atomisation atomic absorption spectrometry

• Published in: Journal of trace elements in medicine and biology 2002-01, Vol.16 (4), p.221-225
• Main Authors: Aguilar, María Victorina, Mateos, Carmen José, Martínez Para, María Carmen
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Calibration; cerebrospinal fluid; Cerebrospinal fluid (biochemistry, cytology, circulation, manometry). Intracranial pressure determination; Chromium - cerebrospinal fluid; chromium determination; electrothermal atomization atomic absorption spectrometry; Hot Temperature; Humans; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Sensitivity and Specificity; Solvents; Spectrophotometry, Atomic - methods
• ISSN: 0946-672X; 1878-3252
• DOI: 10.1016/S0946-672X(02)80048-7

A rapid method to determine the chromium content of cerebrospinal fluid (CSF) samples using electrothermal atomization atomic absorption spectrometry (ETA-AAS) with deuterium-arc background correction is described. The chromium concentration in CSF was evaluated by the standard addition method. Sample dilution (1 + 1) with 0.25% (m/v) Triton X-100 and 4.5% (v/v) HNO 3 gave the best combination of sensitivity, reproducibility, and low blank reading compared with dilution using other solvents. Within-batch reproducibility was 3.2% for 20 CSF samples, between-batch reproducibility was 4.7%. CSF samples from 43 healthy volunteers collected in a manner designed to avoid contamination yielded chromium concentrations of 14.6 ± 6.3 ng mL −1.