The involvement of active DNA synthesis in camptothecin-induced G2 arrest: altered regulation of p34cdc2/cyclin B

Cell cycle arrest in G2 phase is a common response to a variety of DNA-damaging agents. The coupling between DNA damage and G2 arrest was studied in synchronized HeLa cells using camptothecin, a highly specific inhibitor of topoisomerase I that damages DNA through the formation of reversible topoiso...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1992-04, Vol.52 (7), p.1823-1829
Main Authors: Tsao, Y P, D'Arpa, P, Liu, L F
Format: Article
Language:English
Subjects:
Aphidicolin - pharmacology
Camptothecin - pharmacology
CDC2 Protein Kinase - metabolism
Cyclins - metabolism
Dimethyl Sulfoxide - pharmacology
DNA Damage
DNA Replication
DNA Topoisomerases, Type I - metabolism
G2 Phase - drug effects
HeLa Cells
Humans
Kinetics
Methionine - metabolism
Models, Biological
Neoplasm Proteins - biosynthesis
Neoplasm Proteins - isolation & purification
Protamine Kinase - metabolism
S Phase - drug effects
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Summary:Cell cycle arrest in G2 phase is a common response to a variety of DNA-damaging agents. The coupling between DNA damage and G2 arrest was studied in synchronized HeLa cells using camptothecin, a highly specific inhibitor of topoisomerase I that damages DNA through the formation of reversible topoisomerase I-DNA cleavable complexes. Brief camptothecin treatment of early S-phase HeLa cells caused arrest at G2 phase and abolished the activation of p34cdc2 protein kinase. Both tyrosine dephosphorylation of p34cdc2 and cyclin B accumulation were altered. These cell cycle-dependent changes were not observed when DNA replication was inhibited by aphidicolin during the brief camptothecin treatment. Our results suggest that to produce G2 arrest, active DNA synthesis is required at the time of camptothecin treatment, as was previously shown for camptothecin-induced cytotoxicity. Furthermore, our results suggest that the interaction of the replication fork with DNA damage may ultimately trigger altered regulation of p34cdc2/cyclin B, leading to cell cycle arrest at the G2 phase.
ISSN:0008-5472