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Detection by DGGE of a new polymorphism closely linked to the adenomatous polyposis coli region

The EF5.44 locus is in close proximity to the chromosome 5 region to which the genetic defect responsible for familial adenomatous polyposis has been mapped. We have devised two oligonucleotides that promote the specific polymerase chain reaction (PCR) amplification of a 365-bp sequence in this regi...

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Bibliographic Details
Published in:Human genetics 1992-03, Vol.88 (6), p.658-660
Main Authors: OLSCHWANG, S, FABRE, R, LAURENT-PUIG, P, VASSAL, A, HAMELIN, B, NAKAMURA, Y, THOMAS, G
Format: Article
Language:English
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Summary:The EF5.44 locus is in close proximity to the chromosome 5 region to which the genetic defect responsible for familial adenomatous polyposis has been mapped. We have devised two oligonucleotides that promote the specific polymerase chain reaction (PCR) amplification of a 365-bp sequence in this region. Analysis by denaturing gradient gel electrophoresis of the resulting fragment has unravelled individual differences that could be identified as a single base pair change in a MnlI restriction site. This PCR assayable polymorphism increases the informativeness at this locus, and should be useful in the presymptomatic diagnosis of familial adenomatous polyposis.
ISSN:0340-6717
1432-1203
DOI:10.1007/BF02265293