Effect of ploidy on transcription levels in cultured rat aortic smooth muscle cells

Aging and hypertension increase the number of polyploid smooth muscle cells (SMC) in a blood vessel. We assessed the effect of ploidy on the transcription of several genes in SMC cultures derived from newborn and adult rats. In diploid and tetraploid subcultures of SMC from newborn rats, RNA express...

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Bibliographic Details
Published in:FEBS letters 1992-02, Vol.297 (1), p.189-195
Main Authors: van Neck, Johan W., van Berkel, Patrick H.C., Telleman, Pieter, Steijns, Linda S.W., Onnekink, Carla, Bloemers, Henri P.J.
Format: Article
Language:English
Subjects:
Actins - genetics
Animals
Atherosclerosis
Biological and medical sciences
Blotting, Southern
Cells, Cultured
Collagen - genetics
FCM
Fibronectins - genetics
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Male
Metaphase
Molecular and cellular biology
Molecular genetics
Muscle, Smooth, Vascular - cytology
Muscle, Smooth, Vascular - metabolism
Nucleic Acid Hybridization
PBS
phosphate buffered saline
Platelet-Derived Growth Factor - genetics
Ploidies
Ploidy
Rat
Rats
Rats, Inbred WKY
RNA - metabolism
RNA Probes
SMC
smooth muscle cell(s)
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
Vascular smooth muscle cell
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Summary:Aging and hypertension increase the number of polyploid smooth muscle cells (SMC) in a blood vessel. We assessed the effect of ploidy on the transcription of several genes in SMC cultures derived from newborn and adult rats. In diploid and tetraploid subcultures of SMC from newborn rats, RNA expression of the genes assayed is linked with ploidy. However, when phenotypically different SMC cultures derived from newborn and adult rats were compared, transcription levels varied from gene to gene and not linked with the ploidy. Thus, differences in gene expression due to polyploidy are superimposed on those due to other phenotypical features.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(92)80358-N