New C-band protocol by heat denaturation in the presence of formamide

C‐banding techniques detect the presence of constitutive heterochromatin, which is usually located in centromeric regions of chromosomes in the majority of analysed species. The common method for C‐banding used over the last 30 years involves treatment with a mild alkali barium hydroxide 5 % Ba(OH)2...

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Bibliographic Details
Published in:Hereditas 2002-11, Vol.137 (2), p.145-148
Main Authors: FERNÁNDEZ1, R., BARRAGÁN2, M. J. L., BULLEJOS2, M., MARCHAL2, J. A., DÍAZ DE LA GUARDIA3, R., SÁNCHEZ2, A.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Chromosome Banding - methods
DNA Primers
Formamides - chemistry
Hot Temperature
Humans
Protein Denaturation
Rodentia - genetics
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Summary:C‐banding techniques detect the presence of constitutive heterochromatin, which is usually located in centromeric regions of chromosomes in the majority of analysed species. The common method for C‐banding used over the last 30 years involves treatment with a mild alkali barium hydroxide 5 % Ba(OH)2 at 50°C for 5–15 min and subsequent incubation in salt solution (2×SSC at 60°C for 1 h). We here present a new, easy and reliable technique for C‐banding, which basically involves heat denaturation of chromosomal DNA in the presence of formamide and incubation in 2×SSC at room temperature.
ISSN:0018-0661
1601-5223
DOI:10.1034/j.1601-5223.2002.01672.x