Optimization and validation of an analytical procedure by high-performance liquid chromatography for the quantification of peroxisomicines and isoperoxisomicines

Peroxisomicine A1 is a potential antineoplastic substance extracted from plants of the genus Karwinskia. An RP-HPLC–DAD method was developed and validated for the separation and quantification of four isomers of this compound. These isomers coelute in the preparative procedure and are present at a p...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2003-01, Vol.783 (1), p.85-92
Main Authors: Osorio-Pérez, A, Salazar-Cavazos, M, Piñeyro-López, A, Waksman de Torres, N
Format: Article
Language:English
Subjects:
Anthracenes - analysis
Chromatography, High Pressure Liquid - methods
Isoperoxisomicines
Peroxisomicines
Reproducibility of Results
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Summary:Peroxisomicine A1 is a potential antineoplastic substance extracted from plants of the genus Karwinskia. An RP-HPLC–DAD method was developed and validated for the separation and quantification of four isomers of this compound. These isomers coelute in the preparative procedure and are present at a proportion ranging between 3 and 5% in the peroxisomicine A1 purified in the laboratory. The desirability coefficient of the method described here was enhanced 140% with respect to the previously reported method.
ISSN:1570-0232
1873-376X
DOI:10.1016/S1570-0232(02)00491-9