Cancer cell-motility-inhibitory protein in the dunning adenocarcinoma model

Cell motility has been associated with metastatic ability in the Dunning R3327 rat prostatic adenocarcinoma model. Cancer cell motility promoters but not inhibitors have been described by many investigators. Serum-containing and serum-free media conditioned by the nonmotile, nonmetastatic G Dunning...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1992-04, Vol.52 (8), p.2349-2352
Main Authors: MOHLER, J. L, BROSKIE, E. N, RANPARIA, D. J, SHARIEF, Y, COLEMAN, W. B, SMITH, G. J
Format: Article
Language:English
Subjects:
Adenocarcinoma - chemistry
Animal tumors. Experimental tumors
Animals
Biological and medical sciences
Cell Movement
Electrophoresis, Polyacrylamide Gel
Experimental renal and urinary tract tumors
Male
Medical sciences
Molecular Weight
Neoplasm Proteins - chemistry
Neoplasm Proteins - isolation & purification
Prostatic Neoplasms - chemistry
Rats
Tumor Cells, Cultured
Tumors
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Summary:Cell motility has been associated with metastatic ability in the Dunning R3327 rat prostatic adenocarcinoma model. Cancer cell motility promoters but not inhibitors have been described by many investigators. Serum-containing and serum-free media conditioned by the nonmotile, nonmetastatic G Dunning subline inhibited the motility of the highly motile, highly metastatic MAT-LyLu subline. Motility inhibition by the G subline-conditioned serum-free media was lost upon heating to 100 degrees C and by treatment with trypsin. The motility inhibitory protein(s) had a molecular weight exceeding 50,000 as determined by diafiltration. G subline-conditioned RPMI 1640 contained several proteins with molecular weights of between approximately 53,000 and 116,000 when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. One of these bands may represent the first inhibitor of cancer cell motility identified.
ISSN:0008-5472
1538-7445