The major salivary gland antigens of Culex quinquefasciatus are D7-related proteins

The sera of persons with strong allergic responses to the bites of the mosquito, Culex quinquefasciatus, contained IgE antibodies reactive with two major salivary gland proteins with molecular weights of 35 and 28 kDa. These antigens were purified, their amino termini sequenced, and the sequences we...

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Bibliographic Details
Published in:Insect biochemistry and molecular biology 2003, Vol.33 (1), p.63-71
Main Authors: Malafronte, Rosely dos Santos, Calvo, Eric, James, Anthony A, Marinotti, Osvaldo
Format: Article
Language:English
Subjects:
Adolescent
Adult
Amino Acid Sequence
Animals
Antigens - immunology
Base Sequence
Blotting, Western
Child
Child, Preschool
Culex - immunology
Culex quinquefasciatus
DNA Primers
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunoglobulin E - blood
Immunoglobulin E - immunology
Molecular Sequence Data
Mosquito bite allergy
Salivary glands
Salivary Glands - immunology
Sequence Homology, Amino Acid
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Summary:The sera of persons with strong allergic responses to the bites of the mosquito, Culex quinquefasciatus, contained IgE antibodies reactive with two major salivary gland proteins with molecular weights of 35 and 28 kDa. These antigens were purified, their amino termini sequenced, and the sequences were used to search for similar sequences in public databases. Two cDNAs, CuQu-D7Clu1 and CuQu-D7Clu12, which encode D7-related proteins, were identified as containing predicted amino acid sequences identical to the 35 and 28 kDa antigens, respectively. These proteins are expressed specifically in adult female salivary glands and, their predicted tertiary structures are consistent with a role as carriers of hydrophobic molecules in mosquito saliva.
ISSN:0965-1748
1879-0240
DOI:10.1016/S0965-1748(02)00168-6