A complete cDNA for adenine phosphoribosyltransferase from Arabidopsis thaliana

An intact cDNA from Arabidopsis thaliana for adenine phosphoribosyltransferase (APRT) was isolated and sequenced. The cDNA is 729 nucleotides in length and predicts a protein of Mr 27,140. The deduced amino acid sequence has been compared with those of other APRTs and shown to be most similar to the...

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Bibliographic Details
Published in:Plant molecular biology 1992-02, Vol.18 (4), p.653-662
Main Authors: MOFFATT, B. A, MCWHINNIE, E. A, BURKHART, W. E, PASTERNAK, J. J, ROTHSTEIN, S. J
Format: Article
Language:English
Subjects:
Adenine Phosphoribosyltransferase - genetics
Adenine Phosphoribosyltransferase - metabolism
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Blotting, Northern
DNA
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Genes. Genome
Humans
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Phylogeny
Plant Proteins - genetics
Plants - enzymology
Plants - genetics
Sequence Alignment
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Summary:An intact cDNA from Arabidopsis thaliana for adenine phosphoribosyltransferase (APRT) was isolated and sequenced. The cDNA is 729 nucleotides in length and predicts a protein of Mr 27,140. The deduced amino acid sequence has been compared with those of other APRTs and shown to be most similar to the Escherichia coli protein. Construction of a molecular tree of the known APRT amino acid sequences indicates the A. thaliana and E. coli APRT sequences form one cluster and the currently available vertebrate and invertebrate sequences form a separate grouping. Since it is possible to select either for or against the expression of APRT, the isolation of this APRT cDNA clone will allow these selection schemes to be used in plant genetic experiments.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00020008