DNA methylation inhibits propagation of tomato golden mosaic virus DNA in transfected protoplasts

The effects of methylation on plant viral DNA replication have been studied in Nicotiana tabacum protoplasts transfected with DNA of the geminivirus tomato golden mosaic virus (TGMV). The transfected cells were also used to determine whether experimentally introduced methylation patterns are maintai...

Full description

Saved in:
Bibliographic Details
Published in:Plant molecular biology 1992-02, Vol.18 (4), p.703-712
Main Authors: BROUGH, C. L, GARDINER, W. E, INAMDAR, N. M, ZHANG, X.-Y, EHRLICH, M, BISARO, D. M
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Biotechnology
Blotting, Southern
DNA Replication
DNA, Recombinant
DNA, Viral - biosynthesis
DNA, Viral - metabolism
Fundamental and applied biological sciences. Psychology
Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control
Kinetics
Methylation
Mosaic Viruses - genetics
Nicotiana tabacum
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
Protoplasts - microbiology
Restriction Mapping
tomato golden mosaic virus
Transfection
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The effects of methylation on plant viral DNA replication have been studied in Nicotiana tabacum protoplasts transfected with DNA of the geminivirus tomato golden mosaic virus (TGMV). The transfected cells were also used to determine whether experimentally introduced methylation patterns are maintained in extrachromosomal viral DNA. Replacement of cytosine residues with 5-methylcytosine (m5C) reduced the amount of viral DNA which accumulated in transfected protoplasts. The reduction was observed whether m5C residues were substituted for cytosine residues in vitro in either the viral strand or the complementary strand of double-stranded circular inoculum DNAs containing tandemly repeated copies of the A component of the TGMV genome. Both limited and extensive cytosine methylation of TGMV DNA sequences in vitro was not propagated in progeny viral DNA. The absence of detectable maintenance-type methylation of the transfecting TGMV DNA sequences may be related to the lack of methylation observed in double-stranded TGMV DNA isolated from infected plants.
ISSN:0167-4412
1573-5028
DOI:10.1007/bf00020012