Loading…
A novel solid support for synthesis of oligonucleotide 3′-phosphorothioate monoesters
A new reagent immobilized on solid support allowing for solid-phase synthesis of oligonucleotides with a 3′-terminal phosphorothioate monoester is described. The support is compatible with phosphoramidite chemistry for automated oligonucleotide synthesis. Final deprotection with ammonia under standa...
Saved in:
| Published in: | Bioorganic & medicinal chemistry letters 2003-01, Vol.13 (2), p.281-284 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c457t-fb49daed67b2f1d353fc4a4a1a65599852aff0502cd1a28080f332c8bd02e7e3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c457t-fb49daed67b2f1d353fc4a4a1a65599852aff0502cd1a28080f332c8bd02e7e3 |
| container_end_page | 284 |
| container_issue | 2 |
| container_start_page | 281 |
| container_title | Bioorganic & medicinal chemistry letters |
| container_volume | 13 |
| creator | Cheruvallath, Zacharia S Cole, Douglas L Ravikumar, Vasulinga T |
| description | A new reagent immobilized on solid support allowing for solid-phase synthesis of oligonucleotides with a 3′-terminal phosphorothioate monoester is described. The support is compatible with phosphoramidite chemistry for automated oligonucleotide synthesis. Final deprotection with ammonia under standard conditions leads to oligonucleotide 3′-terminal phosphorothioate.
A new reagent immobilized on solid support allowing for solid-phase synthesis of oligonucleotides with a 3′-terminal phosphorothioate monoester is described. The support is compatible with phosphoramidite chemistry for automated oligonucleotide synthesis. Final deprotection with ammonia under standard conditions leads to oligonucleotide 3′-terminal phosphorothioate monoester (3′-TPT). |
| doi_str_mv | 10.1016/S0960-894X(02)00922-8 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_72888103</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0960894X02009228</els_id><sourcerecordid>72888103</sourcerecordid><originalsourceid>FETCH-LOGICAL-c457t-fb49daed67b2f1d353fc4a4a1a65599852aff0502cd1a28080f332c8bd02e7e3</originalsourceid><addsrcrecordid>eNqFkM1O3DAQx62qCJaPR2jlSxE9pIwdJ3FOCKF-ICH1UKT2ZnntcdcoGwdPgsStz8Qj9UmaZVdw7GE0h_n9Z0Y_xt4J-CRA1Oc_oK2h0K36dQbyI0ArZaHfsIVQtSpKBdVbtnhBDtgh0R2AUKDUPjsQUmmpFCzYz0vepwfsOKUuek7TMKQ88pAyp8d-XCFF4inwefo79ZPrMI3RIy___nkqhlWiuXIaVzHZEfk69QlpxEzHbC_YjvBk14_Y7ZfPt1ffipvvX6-vLm8Kp6pmLMJStd6ir5ulDMKXVRmcssoKW1dV2-pK2hCgAum8sFKDhlCW0umlB4kNlkfsdLt2yOl-mk-bdSSHXWd7TBOZRmqtBZQzWG1BlxNRxmCGHNc2PxoBZiPUPAs1G1sGpHkWavSce787MC3X6F9TO4Mz8GEHWHK2C9n2LtIrp5RsodlwF1sOZxsPEbMhF7F36GNGNxqf4n9e-QdhnpVn</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>72888103</pqid></control><display><type>article</type><title>A novel solid support for synthesis of oligonucleotide 3′-phosphorothioate monoesters</title><source>ScienceDirect Freedom Collection</source><creator>Cheruvallath, Zacharia S ; Cole, Douglas L ; Ravikumar, Vasulinga T</creator><creatorcontrib>Cheruvallath, Zacharia S ; Cole, Douglas L ; Ravikumar, Vasulinga T</creatorcontrib><description>A new reagent immobilized on solid support allowing for solid-phase synthesis of oligonucleotides with a 3′-terminal phosphorothioate monoester is described. The support is compatible with phosphoramidite chemistry for automated oligonucleotide synthesis. Final deprotection with ammonia under standard conditions leads to oligonucleotide 3′-terminal phosphorothioate.
A new reagent immobilized on solid support allowing for solid-phase synthesis of oligonucleotides with a 3′-terminal phosphorothioate monoester is described. The support is compatible with phosphoramidite chemistry for automated oligonucleotide synthesis. Final deprotection with ammonia under standard conditions leads to oligonucleotide 3′-terminal phosphorothioate monoester (3′-TPT).</description><identifier>ISSN: 0960-894X</identifier><identifier>EISSN: 1464-3405</identifier><identifier>DOI: 10.1016/S0960-894X(02)00922-8</identifier><identifier>PMID: 12482440</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Ammonia - chemistry ; Carbohydrates. Nucleosides and nucleotides ; Chemistry ; Electrophoresis, Capillary ; Exact sciences and technology ; Indicators and Reagents ; Magnetic Resonance Spectroscopy ; Nucleosides, nucleotides and oligonucleotides ; Oligonucleotides - chemical synthesis ; Organic chemistry ; Organothiophosphorus Compounds - chemical synthesis ; Preparations and properties</subject><ispartof>Bioorganic & medicinal chemistry letters, 2003-01, Vol.13 (2), p.281-284</ispartof><rights>2002 Elsevier Science Ltd</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-fb49daed67b2f1d353fc4a4a1a65599852aff0502cd1a28080f332c8bd02e7e3</citedby><cites>FETCH-LOGICAL-c457t-fb49daed67b2f1d353fc4a4a1a65599852aff0502cd1a28080f332c8bd02e7e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14429070$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12482440$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cheruvallath, Zacharia S</creatorcontrib><creatorcontrib>Cole, Douglas L</creatorcontrib><creatorcontrib>Ravikumar, Vasulinga T</creatorcontrib><title>A novel solid support for synthesis of oligonucleotide 3′-phosphorothioate monoesters</title><title>Bioorganic & medicinal chemistry letters</title><addtitle>Bioorg Med Chem Lett</addtitle><description>A new reagent immobilized on solid support allowing for solid-phase synthesis of oligonucleotides with a 3′-terminal phosphorothioate monoester is described. The support is compatible with phosphoramidite chemistry for automated oligonucleotide synthesis. Final deprotection with ammonia under standard conditions leads to oligonucleotide 3′-terminal phosphorothioate.
A new reagent immobilized on solid support allowing for solid-phase synthesis of oligonucleotides with a 3′-terminal phosphorothioate monoester is described. The support is compatible with phosphoramidite chemistry for automated oligonucleotide synthesis. Final deprotection with ammonia under standard conditions leads to oligonucleotide 3′-terminal phosphorothioate monoester (3′-TPT).</description><subject>Ammonia - chemistry</subject><subject>Carbohydrates. Nucleosides and nucleotides</subject><subject>Chemistry</subject><subject>Electrophoresis, Capillary</subject><subject>Exact sciences and technology</subject><subject>Indicators and Reagents</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Nucleosides, nucleotides and oligonucleotides</subject><subject>Oligonucleotides - chemical synthesis</subject><subject>Organic chemistry</subject><subject>Organothiophosphorus Compounds - chemical synthesis</subject><subject>Preparations and properties</subject><issn>0960-894X</issn><issn>1464-3405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqFkM1O3DAQx62qCJaPR2jlSxE9pIwdJ3FOCKF-ICH1UKT2ZnntcdcoGwdPgsStz8Qj9UmaZVdw7GE0h_n9Z0Y_xt4J-CRA1Oc_oK2h0K36dQbyI0ArZaHfsIVQtSpKBdVbtnhBDtgh0R2AUKDUPjsQUmmpFCzYz0vepwfsOKUuek7TMKQ88pAyp8d-XCFF4inwefo79ZPrMI3RIy___nkqhlWiuXIaVzHZEfk69QlpxEzHbC_YjvBk14_Y7ZfPt1ffipvvX6-vLm8Kp6pmLMJStd6ir5ulDMKXVRmcssoKW1dV2-pK2hCgAum8sFKDhlCW0umlB4kNlkfsdLt2yOl-mk-bdSSHXWd7TBOZRmqtBZQzWG1BlxNRxmCGHNc2PxoBZiPUPAs1G1sGpHkWavSce787MC3X6F9TO4Mz8GEHWHK2C9n2LtIrp5RsodlwF1sOZxsPEbMhF7F36GNGNxqf4n9e-QdhnpVn</recordid><startdate>200301</startdate><enddate>200301</enddate><creator>Cheruvallath, Zacharia S</creator><creator>Cole, Douglas L</creator><creator>Ravikumar, Vasulinga T</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200301</creationdate><title>A novel solid support for synthesis of oligonucleotide 3′-phosphorothioate monoesters</title><author>Cheruvallath, Zacharia S ; Cole, Douglas L ; Ravikumar, Vasulinga T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-fb49daed67b2f1d353fc4a4a1a65599852aff0502cd1a28080f332c8bd02e7e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Ammonia - chemistry</topic><topic>Carbohydrates. Nucleosides and nucleotides</topic><topic>Chemistry</topic><topic>Electrophoresis, Capillary</topic><topic>Exact sciences and technology</topic><topic>Indicators and Reagents</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Nucleosides, nucleotides and oligonucleotides</topic><topic>Oligonucleotides - chemical synthesis</topic><topic>Organic chemistry</topic><topic>Organothiophosphorus Compounds - chemical synthesis</topic><topic>Preparations and properties</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cheruvallath, Zacharia S</creatorcontrib><creatorcontrib>Cole, Douglas L</creatorcontrib><creatorcontrib>Ravikumar, Vasulinga T</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Bioorganic & medicinal chemistry letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cheruvallath, Zacharia S</au><au>Cole, Douglas L</au><au>Ravikumar, Vasulinga T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel solid support for synthesis of oligonucleotide 3′-phosphorothioate monoesters</atitle><jtitle>Bioorganic & medicinal chemistry letters</jtitle><addtitle>Bioorg Med Chem Lett</addtitle><date>2003-01</date><risdate>2003</risdate><volume>13</volume><issue>2</issue><spage>281</spage><epage>284</epage><pages>281-284</pages><issn>0960-894X</issn><eissn>1464-3405</eissn><abstract>A new reagent immobilized on solid support allowing for solid-phase synthesis of oligonucleotides with a 3′-terminal phosphorothioate monoester is described. The support is compatible with phosphoramidite chemistry for automated oligonucleotide synthesis. Final deprotection with ammonia under standard conditions leads to oligonucleotide 3′-terminal phosphorothioate.
A new reagent immobilized on solid support allowing for solid-phase synthesis of oligonucleotides with a 3′-terminal phosphorothioate monoester is described. The support is compatible with phosphoramidite chemistry for automated oligonucleotide synthesis. Final deprotection with ammonia under standard conditions leads to oligonucleotide 3′-terminal phosphorothioate monoester (3′-TPT).</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>12482440</pmid><doi>10.1016/S0960-894X(02)00922-8</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0960-894X |
| ispartof | Bioorganic & medicinal chemistry letters, 2003-01, Vol.13 (2), p.281-284 |
| issn | 0960-894X 1464-3405 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72888103 |
| source | ScienceDirect Freedom Collection |
| subjects | Ammonia - chemistry Carbohydrates. Nucleosides and nucleotides Chemistry Electrophoresis, Capillary Exact sciences and technology Indicators and Reagents Magnetic Resonance Spectroscopy Nucleosides, nucleotides and oligonucleotides Oligonucleotides - chemical synthesis Organic chemistry Organothiophosphorus Compounds - chemical synthesis Preparations and properties |
| title | A novel solid support for synthesis of oligonucleotide 3′-phosphorothioate monoesters |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T17%3A36%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20novel%20solid%20support%20for%20synthesis%20of%20oligonucleotide%203%E2%80%B2-phosphorothioate%20monoesters&rft.jtitle=Bioorganic%20&%20medicinal%20chemistry%20letters&rft.au=Cheruvallath,%20Zacharia%20S&rft.date=2003-01&rft.volume=13&rft.issue=2&rft.spage=281&rft.epage=284&rft.pages=281-284&rft.issn=0960-894X&rft.eissn=1464-3405&rft_id=info:doi/10.1016/S0960-894X(02)00922-8&rft_dat=%3Cproquest_cross%3E72888103%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c457t-fb49daed67b2f1d353fc4a4a1a65599852aff0502cd1a28080f332c8bd02e7e3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=72888103&rft_id=info:pmid/12482440&rfr_iscdi=true |