Complete Freund's adjuvant immunization prolongs survival in experimental prion disease in mice

We recently reported that immunization of mice with certain self‐prion protein peptides induced specific T‐cell and B‐cell immune responses; importantly, this immunization was associated with a decrease in the number of protease‐resistant PrPSc particles recoverable in a transplanted, scrapie‐infect...

Full description

Saved in:
Bibliographic Details
Published in:Journal of neuroscience research 2003-01, Vol.71 (2), p.286-290
Main Authors: Tal, Yuval, Souan, Lina, Cohen, Irun R., Meiner, Zeev, Taraboulos, Albert, Mor, Felix
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Blotting, Western
Disease Models, Animal
Female
Freund's adjuvant
Freund's Adjuvant - immunology
Freund's Adjuvant - therapeutic use
Immunization
immunotherapy
Mice
Mice, Inbred C57BL
Peptide Fragments - immunology
Peptide Fragments - pharmacology
Prion Diseases - immunology
Prion Diseases - mortality
Prion Diseases - therapy
Prions - drug effects
Prions - metabolism
Protein Isoforms - chemistry
Protein Isoforms - metabolism
PrPSc
PrPSc Proteins - metabolism
scrapie
Scrapie - metabolism
Survival Rate
Time Factors
transmissible spongiform encephalopathies
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We recently reported that immunization of mice with certain self‐prion protein peptides induced specific T‐cell and B‐cell immune responses; importantly, this immunization was associated with a decrease in the number of protease‐resistant PrPSc particles recoverable in a transplanted, scrapie‐infected syngeneic tumor. The present study was carried out to determine whether immunization with the immunogenic PrP peptides might influence the natural history of experimental scrapie in mice. We immunized C57BL/6 mice with self‐prion peptides in complete Freund's adjuvant (CFA) or with CFA alone as a control and then infected the mice with mouse‐adapted scrapie by injection either intraperitoneally or intracerebrally. We report here that immunization with CFA, irrespective of whether prion peptides were present in the inoculum, resulted in marked prolongation of survival of the mice, whether the challenge was intracerebral or intraperitoneal. Mice in the immunized and control groups that died contained equivalent amounts of PrPSc. Thus, CFA immunization has a therapeutic effect in experimental scrapie in mice, possibly by reducing the rate of PrPSc accumulation in the brain. © 2002 Wiley‐Liss, Inc.
ISSN:0360-4012
1097-4547
DOI:10.1002/jnr.10474