GROWTH OF AN OVARIAN CELL LINE OF GALLERIA MELLONELLA AND ITS RESPONSE TO IMMUNE-INDUCING FACTORS

Antibacterial proteins are produced in the reproductive tracts of some insect species. The advent of a pupal ovarian cell line of the lepidopteran Galleria mellonella offered an opportunity for exploring the use of ovarian tissue culture to induce antimicrobial proteins in lieu of the larvae. The ov...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 2002-11, Vol.38 (10), p.572-581
Main Authors: ZAKARIAN, ROBERT J, DUNPHY, GARY B, QUIOT, JEAN-MARIE
Format: Article
Language:English
Subjects:
20-hydroxyecdysone
Animals
apolipophorin-III
Bacillus subtilis
Bacteria
Cattle
Cell Culture Techniques - methods
Cell Division - drug effects
Cell growth
CELL GROWTH/DIFFERENTIATION/APOPTOSIS
Cell Line
Cell lines
Culture Media
Cultured cells
Female
Galleria
Galleria mellonella
Hemocytes
Insect biochemistry
Insect genetics
Insect larvae
Insect reproduction
Insect vectors
Kinetics
Larva
Lepidoptera
morphogenesis
Moths - cytology
Moths - growth & development
ovarian
Ovary - cytology
Ovary - drug effects
Pupa
Sucrose - pharmacology
Temperature
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Summary:Antibacterial proteins are produced in the reproductive tracts of some insect species. The advent of a pupal ovarian cell line of the lepidopteran Galleria mellonella offered an opportunity for exploring the use of ovarian tissue culture to induce antimicrobial proteins in lieu of the larvae. The ovarian cell growth rates and cell yields were maximized by adjusting Grace's medium to pH 6.5, adding 15% (v/v) qualified heat-inactivated fetal calf serum, and lowering the sucrose concentration to 9.3 g/L. Five cell forms and biochemical profiles of the collective cell types were analyzed throughout the culture growth cycle. The final modified culture medium did not affect morphogenesis, whereas it increased the culture growth rate by 50% and the final cell yield threefold. The molting and immunoprotein-inducing hormone, 20-hydroxyecdysone, increased culture growth rate and altered the levels of cell types A and D. Neither 20-hydroxyecdysone nor the larval immunizing agents, apolipophorin-III or Bacillus subtilis, in combination or alone, induced antibacterial activity. The bacterium did induce immunity in both larval and adult stages.
ISSN:1071-2690
1543-706X
1543-706X
DOI:10.1290/1543-706X(2002)38<572:GOAOCL>2.0.CO;2